对钙调神经磷酸酶有激活作用的火麻仁提取物改善化学药品诱发的小鼠学习记忆功能障碍.pdfVIP

维普资讯 LuoJ etal／ActaPharmacolSin 2003Nov；24(11)：1137—1142 l137 @2003，ActaPharmacologicaSinica ChinesePharmacologicalSociety ShanghaiInstituteofMateriaMedica ChineseAcademyofSciences http：／／www ChinaPhar．com Extractfrom Fructuscannabisactivatingcalcineurin ● 1 1 - _ improvedlearmngandmemory 一 一 v inmicewithchemicaldrug—induceddysmnesia1 LUOJing，YINJiang—Hua，WUHe—Zhen，WEIQun DepartmentofBiochemistryandMolecularBiology，BeijingNormalUniversity,Beijing100875,China KEY WORDSFructuscannabis；calcineurin；avoidancelearning；mazelearning；memorydisorders AIM ：Toinvestigatetheeffectsofextractrfom FructuscannabisrEFC)thatcanactivatecalcineurinonlearning andmemory impairmentinducedbychemicaldrugsinmi ce．M[ETHoDS：Bovinebraincalcineurinnadcalmodulin wereisolatedrfomrfozentissues．TheactivityofcalcineurinwasassayedusingP—nitrophenylphosphate(PNPP) asthesubstrate．Step—downtypepassiveavoidancetestandwatermazewereusedtogethertodeterminethe effectsofEFConlearningnadmemory dysfunction．RESULTS：EFCactivatedcalcineurinactivityataconcentra— tionrangeof0．01—100g／L．ThemaximaIvalueofEFConcalcineurinactivity(35％±5％1appearedataconcen— trationof10g／L．Thechemicaldurgssuchasscopolamine，sodium nitrite，and45％ ethano1na dsodium pento— ． brabitalinducedlearningandmemor