B细胞型非霍奇金淋巴瘤CHFR基因mRNA表达和启动子区甲基化相关性的分析.pdf

发生甲基化负相关，提示在非霍奇金淋巴瘤的发生过程中，CHFR启动子区甲基化可 能导致了该基因mRNA表达下调或缺失，使CHFR基因对细胞有丝分裂的调控功能失 活，从而导致淋巴瘤的发生；③CHAR基因表达下调及启动子区甲基化在B-NHL患者 的临床分期及IPI评分方面具有差异，提示CHFR基因可能与肿瘤的发生及进展相 关，mRNA表达下调及启动子去甲基化是预后不良的因素。 硕士研究生：高艳华(儿科学) 指导老师：宋爱琴副教授 关键词：B细胞型非霍奇金淋巴瘤；CHFR基因；甲基化；实时荧光定量 PCR；MSP of ofmRNA leveland CHFR Study expressionhypermethylaion B—cell lymphoma promoterNon—Hodgkin’S Abstract and To the betweentheCHFR level objectiveinvestigaterelationship expression aberrant in thecorrelation methylationnon-Hodgldnlymphoma(NHL)，andanalyzed betweenCHFRaberrant and characteristics． expressionclinic—pathological The ofCHFRmRNAweredetectedReal—time Methodsexpression by 8 fluorescence PCRaswellas3 reactive of node(RH) quantitative hyperplasialymph chain usedto samples．Themethylation—specificpolymerasereaction(MSP)was ofCHFR in81 NHL and38RH examinethe status genepromoter patients methylation tissues． was in RH the ofmRNA Results mRNAshownthe level QCHFR group，but was lowerin81B-NHL themRNAwasloss expressionsignificantly examples，and in2 rela