富集培养下渤海积物中油气微生物多样性及其对轻烃降解规律的研究.pdfVIP

养基的溶解作用和沉积物的吸附作用均可被忽略。Cl～C5轻烃的生物降解曲线呈明 显的反“S”型，微生物对不同轻烃的降解作用具有选择性：碳数越少的轻烃组分越容 易发生生物降解，正构烷烃较碳数相同的异构烷烃更容易发生生物降解。研究结果 对于探明海底环境对轻烃的作用机制，认清海洋油气藏的成矿机理，深入了解油气 藏海域生态地质环境中细菌的物质代谢特征及多样性，指导微生物油气勘探具有重 要意义。 关键词：油气微生物；海洋沉积物；多样性：吸附与解吸；生物降解 The and ofthe diversity lighthydrocarbonbiodegradationoil-gas fromthe enriched BohaiSeamarinesediments microorganisms Abstraet The enrichmentof ofnear-surfaceoil leakage lighthydrocarboncomponents formmarine are to depositsmay hydrocarboncomponents oil—gasdeposits．Light prone OCCur and orchemical inthis biochemical absorptionphy’sical adsorptionprocess，and effectwilloccurundersuitableconditions．Different lighthydrocarbonsdegrading bacteriawereenrichedfromthemarinesedimentnearthe field．We oil·gas investigated thebacteria structureinenrichmentsedimentswith16SrDNA community library resultsofthe enrichmentculture archaeaand analysis．Thephylogenetic Cl—C5 degrading bacteriashowthatthelibraries islow．Methanotorrisisfoundinthe biodiversity igneus archaea indicatesthe ofthe homologoussequences，which presence symbiotic between and relationshiphydrocarbondegradationmethanogenic ammonia archaeaindicatesthatmethaneoxidationandammoniaoxidationare oxidizing toeach andRhodobacteralesarethe coupled other．Methylo