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。ψ 3S 80丁 iJ I 们←TL 气￥ - - V A CNIC-c目 110 J 一 牛 牛 iMC-0014 噩噩噩 1.1.‘矗-一一 …』……咱. ?? CHINA. NUCLEAR SCIENα. TECHNOLOGY REPORT GOCoγ射线对伴刀豆球蛋白 Con A 和脂多糖 LPS 诱导的淋巴细胞的效应 !lili--hrlL 岛、 申圃辙惰抱中心 : China Nuclear Information Centre tNlt-00110 8Mι0014 60Co y射线对伴刀豆球蛋白 Con A 和脂多糖 LPS 诱导的淋巴细胞的效应 苏燎原刘克良马祥瑞许玉杰刘芬菊 刘犁许吕韶俞志英姚德元高耀明 芬州医学院 中国核情报中心 北. .1187.1 摘要 应用 IH-TdR 掺入法研究COD A 租 LPS 诱导后的淋巴细胞亚霹的辐射量应及其相互 关系.体外实验说明COD A细胞具有激活LPS对B细跑的诱导作用.当 COD A 细胞曼lOG，. 照射后 I ←TdR 掺入明显下降，并失去激活作用. LPS 细跑受照不能被正常COD A 细跑所 .话.两种细凰受照后混合培养出现COD A 细跑对 LPS 细跑的抑制作用.应用穰酷扩散盒 培养桂反映辐射对两种细跑的效应更明显. 倒鼻咽癌病人接受一个疗理的Coy 统治疗后，其Con A细胞的掺入显著下降，失去 撒话B 细跑的作用. LPS 细且不能被正常COD A 细胞所激活.本实验提示COD A 细跑的 辐射敏感性比 LPS 细凰高. 提.调 Con A 诱导淋巴细胞 LPS 诱导淋巴细胞 IH-TdR 掺入 ·中国自然科学基金资助的课题. THE EFFECT OF lOCO Y-RAYS ON COD A AND LPS INDUCED LYMPHOCYTES Su Liaoyuan L iuKeliang Ma Xiangrui Xu Yujie Liu Fenju Liu Li Xu Changshao Yu Zhiying Yao Deyua :t Gao Yaoming Suzhou Medical College ABSTRACT The errect of Co v-rays on lymphocytes induced by Con A and LPg and the relationship behreen these hro groups of cells were investigated by means of H-TdR incorporation. The study showed that in vitro , Con A cells were able to promote the inducing effect of LPg to B cells. When Con A cells were irradiated by 10 Gy V-rays , the H-TdR inLorporation value reduced significantly and the stimulating effect of Con A cells on LPg cells disappeared. Hning been irradiated by v-rays , LPg cells were not be able to be stimu- lated by normal Con A cells. When the groups of ulls were incubated together after irradiation , the synergistir fundion disappeared. furthermore the sup- pressin errect of Con A cells on LPg cells emerged. When these two gr 唱 。1 cells ,ere investigated by means of agar culture. the suppressive effect of lOGy v-rays on lymphocytes colony fornlation was more