嫁接对温度胁迫辣椒抗性生理指标的影响研究.pdfVIP

摘 要 本试验选用野生辣椒品种“威壮贝尔”作砧木，分别在光照培养箱和温室中研 究了辣椒及其嫁接后低温对其苗期叶片、根系和高温对其结果期叶片生理指标的影 响，明确嫁接对辣椒抗温度胁迫的影响，旨在为辣椒耐寒、耐热性栽培及生产提供 依据。结果表明： 1.低温逆境下，较自根苗相比嫁接可降低辣椒叶片和根系 MDA 含量及相对电 导率，减缓超氧阴离子产生速率，从而降低低温下辣椒的膜质过氧化程度，减轻对 细胞膜的伤害。 2.低温逆境下，较自根苗相比嫁接还可提高辣椒叶片和根系中抗氧化保护酶类 （SOD、POD 、CAT ）的活性及渗透调节物质（游离脯氨酸、可溶性蛋白、可溶性 糖）含量且比自根苗多保持2d 左右的高抗氧化保护酶活性和高渗透调节物质含量。 3.综合辣椒幼苗细胞膜受伤害程度、抗氧化酶类活性、渗透调节物质含量方面 的研究，可以得出，嫁接可有效的提高辣椒苗期抗冷的能力，延长辣椒抵御苗期低 温 （昼夜温度为 10℃和5 ℃）2d 左右。 4.高温逆境下，较自根苗相比嫁接可减轻辣椒叶片细胞膜的受损害程度，提高 抗氧化保护酶类的活性和渗透调节物质的含量，说明嫁接可以有效地提高辣椒抵抗 热胁迫的能力。 关键词：嫁接；辣椒；温度胁迫；生理指标 Effect of grafting to pepper stress resistance physiological indexes under temperature stress Abstract This study selected the pepper varieties “Very Strong Bell” as grafted rootstock. Respectively, in light of incubator and greenhouse, the effect of low temperature stress on pepper seeding leaves, roots physiological indicators and high temperature stress on pepper fruiting leaves physiological indicators of self-rooted and grafted pepper were researched. Clear the effect of grafting on Pepper Resistance to temperature stress, so as to provide the basis for pepper cultivation and productionthe under Cold, heat stress. results show that: 1 Under low temperature stress, compared with self-rooted pepper, grafting can reduce the MDA content and the relative conductivity, can slow down the production rate of 02- of pepper seedling leaves and roots, thereby can reducing membrane lipid peroxidation and reducing damage to the cell membrane of the pepper seedling. 2 Under low temperature stress, compared with self-rooted pepper, grafting can also improve the activity of protective antioxidant enzymes (SOD, POD, CAT) and the