Construction, Verification and Experimental Use of Two Epitope-Tagged Collections of Budding Yeast Strains.pdfVIP

Comparative and Functional Genomics Comp Funct Genom 2005; 6: 2–16. Published online in Wiley InterScience (). DOI: 10.1002/cfg.449 Research Paper Construction, verification and experimental use of two epitope-tagged collections of budding yeast strains 1 1# 2 1 2 1 Russell Howson , Won-Ki Huh , Sina Ghaemmaghami , James V. Falvo , Kiowa Bower , Archana Belle , Noah Dephoure1 1 2 1 , Dennis D. Wykoff , Jonathan S. Weissman and Erin K. O’Shea * 1Department of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California at San Francisco, 600 16th Street, Genentech Hall, Room GH-S472D, San Francisco, CA 94143-2240, USA 2 Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California at San Francisco, 600 16th Street, Genentech Hall, Room GH-S472D, San Francisco, CA 94143-2240, USA *Correspondence to: Abstract Erin K. O’Shea, Department of Biochemistry and Biophysics, A major challenge in the post-genomic era is the development of experimental Howard Hughes Medical approaches to monitor the properties of proteins on a proteome-wide level. It would Institute, University of California be particularly useful to systematically assay protein subcellular localization, post- at San Francisco, 600 16th translational modifications and protein–protein interactions, both at steady state and Street, Genentech Hall, Room in response to environmental stimuli. Development of new reagents and methods GH-S472D, San Francisco, CA will enhance our ability to do so efficiently and systematica