Emerging Use of Gene Expression Microarrays in Plant Physiology.pdfVIP

Comparative and Functional Genomics Comp Funct Genom 2003; 4: 216–224. Published online 1 April 2003 in Wiley InterScience (). DOI: 10.1002/cfg.277 Conference Review Emerging use of gene expression microarrays in plant physiology Stan D. Wullschleger* and Stephen P. Difazio Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6422, USA *Correspondence to: Abstract Stan D. Wullschleger, Environmental Sciences Division, Microarrays have become an important technology for the global analysis of gene Oak Ridge National Laboratory, expression in humans, animals, plants, and microbes. Implemented in the context Oak Ridge, Tennessee, of a well-designed experiment, cDNA and oligonucleotide arrays can provide high- 37831-6422, USA. throughput, simultaneous analysis of transcript abundance for hundreds, if not E-mail: wullschlegsd@ thousands, of genes. However, despite widespread acceptance, the use of microarrays as a tool to better understand processes of interest to the plant physiologist is still being explored. To help illustrate current uses of microarrays in the plant sciences, several case studies that we believe demonstrate the emerging application of gene expression arrays in plant physiology were selected from among the many posters and presentations at the 2003 Plant and Animal Genome XI Conference. Based on this survey, microarrays are being used to assess gene expression in plants exposed to the experimental ma