Generation and Analysis of Expressed Sequence Tags from Chimonanthus praecox (Wintersweet) Flowers for Discovering Stress-Responsive and Floral Development-Related Genes.pdfVIP

Hindawi Publishing Corporation Comparative and Functional Genomics Volume 2012, Article ID 134596, 13 pages doi:10.1155/2012/134596 Research Article Generation and Analysis of Expressed Sequence Tags from Chimonanthus praecox (Wintersweet) Flowers for Discovering Stress-Responsive and Floral Development-Related Genes Shunzhao Sui,1 Jianghui Luo,1 Jing Ma,1 Qinlong Zhu,2 Xinghua Lei,3 and Mingyang Li1 1 College of Horticulture and Landscape, Chongqing Engineering Research Center for Floriculture, Key Laboratory of Horticulture Science for Southern Mountainous Regions of Ministry of Education, Southwest University, Chongqing 400715, China 2 College of Life Science, South China Agricultural University, Guangzhou 510642, China 3 Department of Botony, Chongqing Agricultural School, Chongqing 401329, China Correspondence should be addressed to Mingyang Li, limy@ Received 16 September 2011; Revised 26 December 2011; Accepted 26 December 2011 Academic Editor: Jinfa Zhang Copyright © 2012 Shunzhao Sui et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. A complementary DNA library was constructed from the flowers of Chimonanthus praecox, an ornamental perennial shrub blossoming in winter in China. Eight hundred sixty-seven high-quality expressed sequence tag sequences with an average read length of 673.8 bp were acquired. A nonredundant set of 479 unigenes, including 94 contigs and 385 singletons, was identified after the expressed sequence tags were clustered and assembled. BLAST analysis against the nonredundant protein database and nonredundant nucleotide database revealed that 405 unigenes shared significan