Genome-Wide Analysis of the Effects of Heat Shock on a Saccharomyces cerevisiae Mutant With a Constitutively Activated cAMP-Dependent Pathway.pdfVIP

Comparative and Functional Genomics Comp Funct Genom 2004; 5: 419–431. Published online in Wiley InterScience (). DOI: 10.1002/cfg.415 Research Article Genome-wide analysis of the effects of heat shock on a Saccharomyces cerevisiae mutant with a constitutively activated cAMP-dependent pathway 1 2 3# 2 2 4 Dawn L. Jones , June Petty , David C. Hoyle , Andrew Hayes , Stephen G. Oliver , Isabel Riba-Garcia , 4 1 Simon J. Gaskell and Lubomira Stateva * 1Department of Biomolecular Sciences, UMIST, PO Box 88, Manchester M60 1QD, UK 2School of Biological Sciences, University of Manchester, The Michael Smith Building, Oxford Road, Manchester M13 9PT, UK 3Department of Computer Science, University of Manchester, Oxford Road, Manchester M13 9PT, UK 4Michael Barber Centre for Mass Spectrometry, Department of Chemistry, UMIST, PO Box 88, Manchester M60 1QD, UK *Correspondence to: Abstract Dr Lubomira Stateva, Department of Biomolecular We have used DNA microarray technology and 2-D gel electrophoresis combined with Sciences, UMIST, PO Box 88, mass spectrometry to investigate the effects of a drastic heat shock from 30 ◦ C to 50 ◦ C Manchester M60 1QD, UK. on a genome-wide scale. This experimental condition is used to differentiate between E-mail: wild-type cells and those with a constitutively active cAMP-dependent pathway in lubomira.stateva@umist.ac.uk Saccharomyces cerevisiae. Whilst more than 50% of the former survive this shock, # almost all of the latter lose viability. We compared the transcriptomes of the wild- Current