Immune Response of Bos indicus Cattle against the Anti-Tick Antigen Bm91 Derived from Local Rhipicephalus (Boophilus) microplus Ticks and Its Effect on Tick Reproduction under Natural Infestation.pdfVIP

Hindawi Publishing Corporation Journal of Parasitology Research Volume 2012, Article ID 907607, 9 pages doi:10.1155/2012/907607 Research Article Immune Response of Bos indicus Cattle against the Anti-Tick Antigen Bm91 Derived from Local Rhipicephalus (Boophilus) microplus Ticks and Its Effect on Tick Reproduction under Natural Infestation Christian Lambertz,1 Natthaphon Chongkasikit,2 Sathaporn Jittapalapong,3 and Matthias Gauly1 1 Department of Animal Science, Georg-August University, Albrecht-Thaer-Weg 3, 37075 Gottingen, Germany ¨ 2 Department of Animal and Aquatic Science, Chiang Mai University, Chiang Mai 50200, Thailand 3 Department of Parasitology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10903, Thailand Correspondence should be addressed to Christian Lambertz, clamber2@gwdg.de Received 2 July 2012; Revised 16 October 2012; Accepted 19 October 2012 Academic Editor: Wej Choochote Copyright © 2012 Christian Lambertz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Antigens of anti-tick vaccines are more efficacious for homologous challenge with local tick strains. cDNA clones encoding for Bm91 from local Rhipicephalus (Boophilus) microplus strains were developed to immunize Bos indicus cattle under field conditions. Three groups of six animals each were injected with the antigen Bm91, saline, and adjuvant, respectively. Animals were immunized three times at 3-week intervals and a fourth time after six months. The anti-Bm91 antibody level, measured