Impedance-Based Miniaturized Biosensor for Ultrasensitive and Fast Prostate-Specific Antigen Detection.pdfVIP

Hindawi Publishing Corporation Journal of Sensors Volume 2011, Article ID 983752, 7 pages doi:10.1155/2011/983752 Research Article Impedance-Based Miniaturized Biosensor for Ultrasensitive and Fast Prostate-Specific Antigen Detection Ganna Chornokur,1, 2, 3 Sunil K. Arya,1 Catherine Phelan,2, 3 Richard Tanner,2 and Shekhar Bhansali1 1 BioMEMS and Microsystems Laboratory, University of South Florida, 4202 East Fowler Avenue, Tampa, FL 33620, USA 2 Moffi tt Cancer Center and Research Institute, 12902 Magnolia Dr., Tampa, FL 33612, USA 3 The Center for Equal Health, University of South Florida, Tampa, FL 33612, USA Correspondence should be addressed to Shekhar Bhansali, bhansali@ Received 8 September 2010; Accepted 25 January 2011 Academic Editor: Michele Penza Copyright © 2011 Ganna Chornokur et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper reports the successful fabrication of an impedance-based miniaturized biosensor and its application for ultrasensitive Prostate-Specific Antigen (PSA) detection in standard and real human plasma solution, spiked with different PSA concentrations. The sensor was fabricated using photolithographic techniques, while monoclonal antibodies specific to human PSA were used as primary capture antibodies. Electrochemical impedance spectroscopy (EIS) was employed as a detection technique. The sensor exhibited a detection limit of 1 pg/ml for PSA with minimal nonspecific binding (NSB). This detection limit is an order of magnitude lower than commercial PSA ELISA assays available on the market. The sensor can be easily modified into an array for