Measurement of the cytoplasmic pH of Dictyostelium discoideum using a low light level microspectrofluorometer.PDFVIP

Measurement of the Cytoplasmic pH of Dictyostelium discoideum Using a Low Light Level Microspectrofluorometer Ruth Furukawa,*~ John E. Wampler,* and Marcus FechheimerS *Department of Biochemistry and ~Department of Zoology, University of Georgia, Athens, Georgia 30602 Abstract. Pyranine was employed as a sensitive pH (Gross, J. D., J. Bradbury, R. R. Kay, M. J. Peacey. indicator in a low light level microspectrofluorometer. 1983. Nature (Lond.). 303:244-245). The theory The in vivo and in vitro s