BMP-7及抑制性Smads在糖尿病肾病发生发展中表达变化.pdfVIP

190 生理学报 Acta Physiologica Sinica, April 25, 2007, 59 (2): 190-196 研究论文 骨形态发生蛋白 -7 及抑制性 Smads 在糖尿病肾病发生发展中 的表达变化 * 杨 勤 ，韩冰，谢汝佳，程明亮 贵阳医学院病理生理学教研室，贵阳 550004 摘 要：本文采用免疫组化、Western blot 及荧光实时定量PCR 方法，动态观察链脲佐菌素(streptozocin, STZ)诱导的大 鼠糖尿病肾病(diabetic nephropathy, DN)发生早期肾脏骨形态发生蛋白-7 (bone morphogenetic protein-7, BMP-7) 、Smad6 、 Smad7 蛋白及mRNA 表达。结果显示，在正常及DN 大鼠肾小管均有BMP-7 、Smad6 、Smad7 蛋白表达，以胞浆表达为 主。DN 大鼠BMP-7 、Smad6 蛋白表达较正常大鼠明显增多(P 0.05) ，且BMP-7 的mRNA 表达呈先增加后降低的状态； 而Smad7 蛋白和mRNA 的表达均呈先增加后降低的状态。转化生长因子-β1 (transforming growth factor-β1, TGF-β1)及Ⅰ型 胶原(collagen type I, COL-I) mRNA 在DN 大鼠肾脏表达较正常大鼠明显增多(P0.05) ，且随着糖尿病进展有逐渐增加的趋 势。结果提示，作为TGF-β超家族信号分子的一员，BMP-7 信号及抑制性Smad 通路在DN 肾纤维化发生早期可能起重要 的反馈性抑制作用。 关键词：糖尿病肾病；骨形态发生蛋白-7 ；Sm ad6 ；Sm ad7 ；转化生长因子- β；链脲佐菌素 中图分类号：R6 92 ；R5 87 .1 ；Q 25 7 Changes of bone morphogenetic protein-7 and inhibitory Smad expression in streptozotocin-induced diabetic nephropathy rat kidney * YANG Qin , HAN Bing, XIE Ru-Jia, CHENG Ming-Liang Department of Pathophysiology, Guiyang Medical College, Guiyang 550004, China Abstract: The present study was designed to observe the expressions of bone morphogenetic protein-7 (BMP-7) and inhibitory Smads in kidney of rats with diabetic nephropathy (DN), and explore the possible mechanism of DN. Male Wistar rats weighing 180-220 g were single injected with streptozocin (STZ, 55 mg/kg body weight) for 2, 4, 8 and 16 weeks to induce DN. Blood glucose, kidney weight/ body weight and 24-hour urine protein in the control and DN rats were examined; the expressions of BMP-7, Smad6 and Smad7 were detected by using immunohistochemical techniques, Western blot and real-time PCR. The results showed that blood glucose and 24- hour urine protein in DN rats were higher than that in the control rats and kidney weight/body weight was also