空肠弯曲杆菌耐性及其主要相关基因的初步分析.pdfVIP

空肠弯曲杆菌耐药性及其主要相关基因的初步分析 摘 要 从20 世纪70 年代起,弯曲杆菌(Campylobacter)在全世界就被普遍认 为是引起肠道感染的主要原因，导致严重的感染和被拖延了的肠炎病 例、败血症都需要抗生素治疗。而随着抗生素的广泛使用，空肠弯曲杆 菌对抗生素的耐药性引起世界的关泛关注，而国内鲜有这方面的报道。 本实验从307 份鸡盲肠内容物样品中分离到47 株空肠弯曲杆菌， 并测定这些空肠弯曲杆菌耐药性，结果显示对对喹诺酮类药（环丙沙星） 耐药的为31.9%；对氨苄青霉素，克林霉素和红霉素耐药性较高，分别 达到85.1%、83.0%和83.0%；而对痢特灵和庆大霉素则较为敏感，敏感 度分别为85.1%和74.5%。有89.4%的菌株显示多重耐药性。 本实验对47 株空肠弯曲杆菌进行RAPD 分型，得到5 条主条带， 分子量在 100~1500bp 之间，依据该引物扩增产物的电泳谱的差异，将 47 株空肠弯曲杆菌区分为6 个型别Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ、Ⅵ。其中Ⅱ型 为主株，共14 株，占总数的29.8% ；Ⅰ型株10，占21.3% ；Ⅲ型9 株， 占19.1%；Ⅳ型9 株，占19.1%；V 型4 株，占8.5%；Ⅵ型1 株，占2.1% 。 本实验没有发现空肠弯曲杆菌基因型与其耐药性之间有明显联系。 本实验利用MAMA PCR 技术，对获得的47 株空肠弯曲杆菌进行检测， 结果显示药敏实验检测所得对环丙沙星耐药的 15 株空肠弯曲杆菌均能 通过MAMA PCR 检测出其在gyrA 基因258 位发生点突变，30 株对喹诺酮 III 药物敏感的菌株均没有检测出突变。检测正确率高达100%。另外两株对 环丙沙星中介的菌株，有一株检测出点突变，经测序验证该点突变确实 存在。 关键字:空肠弯曲杆菌, 基因分型, 耐药性,MAMA PCR IV STUDY ON ANTIMICROBIAL RESISITANCE OF C.JEJUNI AND ITS RELATED GENE Abstract Since the 1970s, C.jejuni has been generally considered as the main cause leading to the intestinal infection throughout the world. Antibiotics are used when Severe infection and delayed enteritis occurred. With widely use of the antibiotics, the Antimicrobial Resistance of C.jejuni is worldwide focused, while few researches have been reported in China. In our study, 47 C.jejuni was isolated from 307 fowl appendix contents, and Antimicrobial Resistance of these bacterium was tested by disk diffusion technique. The result indicated that 31.9% c.jejuni demonstrated resistance to Ciprofloxacin, the high level of resistance seen were to ampicillin (85.1%), Clindamycin (83.0%), and erythromycin (83.0%), and c.jejuni were more sensitive to furaxone (85.1%)and gentamicin(74.5%). Combined resistance was observed on 89.4% c.jejuni. Genoty