马铃薯连作条件的土壤微生物群落变化.pdf

werewerewithwithwereworkedwerewerewerewithwaswithwaswithwithABSTRACTAcidobacteriaWasWasmonoculturemonoculture．InmicrobialmonoculturemonoculturemolecularmonoculturemonoculturemoremicrobesABSTRACTmicrobialmicrobialmonoculturemicrobialmethodsnobeusedusedbacteriabacteriabacteriabacterialbacterialFusariumbetweennotnothavehaveThisareasSOaaarolesnon-monocultureresolution．FewresearchresultsresultsrelativebetweenbetweenbetweenonoroveronoroveroverGemmatimonadetes．Chloroflexinon-monocultureSoilandandareand9．ofofofofofofofasassociationtototentoforfocusedfarfollowandandandandandandanddistributeddisturbeddirectedcoulddescribesdeclinedeclinediseasedidclosecorrelatedcorrelatedabundanceabundanceabundancesthethethetaxa．Fewthesetimetreatmentsthethethethattheeachthethethesethetimeelectricaltotalcommunitiescommunitiesdecreasedcommunitiesthethethecollected．MainIIsoilsoil．Assoilsoilstudiessoilsoilsoilsoilsoilssatudiessoilsoilshowedsoilsoilsoilbornesoilshowedsoilstudiesstudieslowlesslinkedinininininincidenceininin1-7increase 目录 目 录 中文摘要………………………………………………………………………………I 。dI。】BSTRACT…．．…．．…．．．………．…．…．…．…．．…．……………．．…．…．…．…．…．…．…．．……．……．．…．．II 引言…………………………………………………………………………………．1 第一章文献综述……………………………………………………………………．3 1．1微生物与农业可持续发展…………………………………………………．3 1．2连作对农业生产的影响……………………………………………………．5 1．3关于连作与微生物群落之间关系的研究进展……………………………．7 1．3．1连作对土壤微生物数量的影响………………………………………7 1．3．2连作对土壤微生物物种构成的影响…………………………………8 1．4关于土传病原菌……………………………………………………………．8 1．4．1马铃薯连作土壤中土传病原菌种类…………………………………9 1．4．2关于马铃薯连作土传病害的防治研究进展…………………………9 第二章材料和方法…………………………………………………………………11 2．1试验技术路线图……………………………………………………………11 2．2田间试验布置………………………………………………………………11 2．3土壤样品的采集以及土壤性质的测定……………………………………12 2 2．4土壤总DNA提取、扩增、测序…………………………………………1 2．5高通量测序(454测序)…………………………………………………13 2．6数据分析……………………………………………………………………13 2．6．1 454测序结果分析