乌珠穆沁羊生长程中肌内结缔组织中ⅰ、ⅲ型胶原蛋白的变化.pdfVIP

摘 要 本研究采用 1 至 18 月龄不同生长阶段乌珠穆沁羊为研究对象，提取其股二 头肌内结缔组织中Ⅰ、Ⅲ型胶原蛋白，并进行SDS电泳鉴定；利用高效液 相色谱测定所提取的Ⅰ、Ⅲ型胶原蛋白中三种主要氨基酸含量，并依据羟脯氨酸 含量计算出Ⅰ、Ⅲ型胶原蛋白的含量，通过SAS 软件分析肌内结缔组织中Ⅰ、Ⅲ 型胶原蛋白随月龄增加的变化规律；利用氨基酸分析仪对不同生长阶段乌珠穆沁 羊肌内结缔组织中 Ⅰ、Ⅲ型胶原蛋白的全氨基酸含量进行测定，分析其生长过程 中肌内结缔组织中Ⅰ、Ⅲ型胶原蛋白的变化。 结果表明，随着乌珠穆沁羊月龄的增加，骨骼肌肌内结缔组织Ⅰ型胶原蛋白 中羟脯氨酸、甘氨酸和脯氨酸含量均显著增加 （P0.05），而Ⅲ型胶原蛋白中甘 氨酸和脯氨酸含量显著下降 （P0.05），羟脯氨酸含量显著增加 （P0.05）。Ⅰ、 Ⅲ型胶原蛋白的含量随着月龄的增加而显著增加（P0.05）。氨基酸分析结果表 明，随着乌珠穆沁羊月龄的增加，肌内结缔组织中 Ⅰ型胶原蛋白总氨基酸的含量 显著增加（P0.05），必需氨基酸总量则显著下降 （P0.05），而Ⅲ型胶原蛋白总 氨基酸的含量随月龄增加显著下降（P0.05），其中必需氨基酸总量也随月龄的 增加显著下降 （P0.05）；不同生长阶段乌珠穆沁羊肌内结缔组织中Ⅰ、Ⅲ型胶 原蛋白的各种氨基酸含量及所占百分比变化几乎无差异。 关键词：乌珠穆沁羊；肌内结缔组织； Ⅰ型胶原蛋白；Ⅲ型胶原蛋白；氨基酸 Changes of Type Ⅰand Type Ⅲ Collagen in Intramuscular Connective Tissue during Growth of Ujumqin Sheep Abstract Ujumqin sheep, aged from 1 to 18 months, were employed in this study. The type Ⅰand Ⅲ collagen of intramuscular connective tissue was extracted and identified by SDS, also the three major amino acids was determined by high performance liquid chromatographic (HPLC), then the content of type Ⅰand Ⅲ collagens were calculated based on hydroxyproline. The changes of type Ⅰand Ⅲ collagens in intramuscular connective tissue during growth of Ujumqin sheep were analyzed by SAS software. Amino acid analyzer was used to determine the contents of all amino acids, and analyzed the changes of type Ⅰand Ⅲ collagens in intramuscular connective tissue during growth of Ujumqin Sheep. The results showed that three amino acids contents of type Ⅰcollagen in intramuscular connective tissues were increased （P0.05 ）with growth of Ujumqin Sheep, whereas the glycine and proline contents in type Ⅲ collagen were decreased, and the hydroxyproline content w