c-Fos参与羊驼小肠黏膜淋巴细胞生发中心的调控.pdfVIP

中国农业科学 2008,41(10):3300-3304 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2008.10.050 c-Fos参与羊驼小肠黏膜淋巴细胞生发中心的调控 贺俊平，董常生，游蓉丽，谢建山 （山西农业大学动物科技学院，山西太谷 030801 ） 摘要：【目的】研究转录因子 c-Fos 与凋亡相关蛋白 Caspase3 在羊驼小肠黏膜淋巴组织的表达，探索 c-Fos 与羊驼小肠黏膜淋巴细胞生发中心的关系。【方法】用免疫组织化学技术研究 c-Fos 蛋白与 Caspase3 蛋白在羊驼 十二指肠和空肠淋巴组织的表达。【结果】免疫组织化学结果显示羊驼十二指肠黏膜淋巴细胞较少，c-Fos蛋白主 要表达于空肠黏膜下淋巴组织生发中心，在黏膜下淋巴组织向固有层淋巴组织过渡区域有散在表达，在固有层弥 散淋巴组织中c-Fos阳性表达细胞较少。Caspase3蛋白主要表达于空肠黏膜固有层淋巴组织，在黏膜下淋巴组织 生发中心很少表达，在黏膜下淋巴组织向固有层弥散淋巴组织过渡区域 Caspase3 有散在表达。【结论】c-Fos 参 与羊驼空肠黏膜下淋巴组织生发中心增殖与分化的调控。 关键词：羊驼；黏膜免疫；细胞凋亡；c-Fos；Caspase3 c-Fos Involvement in the Regulation of Mucosa Lymphocyte Germinal Center of Small Intestine in Alpaca (lama pacos) HE Jun-ping, DONG Chang-sheng, YOU Rong-li, XIE Jian-shan (College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, Shanxi) Abstract: 【Objective 】 To study the expression of c-Fos and Caspase3 in small intestine lymphoid tissue of alpaca, and to explore the function of c-Fos in the regulation of mucosa lymphocyte germinal center of alpaca small intestine. 【Method 】The distribution of c-Fos and Caspase3 in small intestine lymphoid tissue in alpaca were studied by immunohistochemistry in the present study. 【Result 】 The results showed that few lymphocyte was found in the mucosa of duodenum, the c-Fos positive lymphocytes were located mainly in the germinal center of jejunum Peyers patches, a little c-Fos positive cells were found in the lamina propria of jejunum and c-Fos positive cells could be seen in the region of adjacent lamina propria. The expression of Caspase3 was focused in the lamina propria lymphocytes. There were almost no Caspase3 positive cells in the germi