2D-DIGE技术研究自交不亲和杏品种‘新世纪’花柱表达蛋白.pdfVIP

中国农业科学 2011,44(4):789-797 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2011.04.017 2D-DIGE技术研究自交不亲和杏品种 ‘新世纪’花柱表达蛋白 曹晓艳，冯建荣，王大江，白 茹，刘月霞 （新疆石河子大学农学院，新疆石河子 832003 ） 摘要：【目的】从蛋白质组水平探索杏树自交不亲和的分子遗传机制。【方法】采用双向差异凝胶电泳和质谱 检测技术，对自交不亲和杏品种‘新世纪’在小蕾期、大蕾期、自花授粉后 24 h 及异花授粉（ ‘新世纪’ב巴 旦水杏’）后 24 h 的花柱进行差异性蛋白质组学研究。【结果】在‘新世纪’杏的小蕾期、大蕾期、自花授粉后 24 h、异花授粉 24 h 后的花柱中共检测到约 1 500 个蛋白点，其中有 66 个差异表达的蛋白点。自交不亲和杏柱 头对自体与异体花粉感应在蛋白质组水平有差异，其中异花授粉后花柱中特异表达的蛋白点 1 个，异花授粉后表 达丰度明显升高的蛋白点 4 个，表达丰度明显降低的蛋白点 29 个；质谱分析只有 13 个蛋白质点得到归属鉴定。 【结论】明确‘新世纪’杏花花柱对自体与异体花粉感应在蛋白质组水平上有差异。 关键词：杏；自交不亲和；花柱；蛋白质组 Reaserch of Protein Expression of Style In Self-Incompatibility Cultivar Prunus armeniaca L. cv. Xinshiji by 2D-DIGE Technique CAO Xiao-yan, FENG Jian-rong, WANG Da-jiang, BAI Ru, LIU Yue-xia (College of Agriculture，Shihezi University ，Shihezi 832003 ，Xinjiang ) Abstract: 【Objective 】 The objective of the experiment is to explore self-incompatibility (SI) molecular mechanism of apricot at proteome level. 【Method 】Different proteomics of style at the stage of the small bud, big bud, 24 h after self-pollination (ASP) and 24 h after cross-pollination (ACP) (‘Xinshiji’בBadanshui’) in the SI apricot ‘Xinshiji’ by two dimension difference gel electrophoresis (2D-DIGE) and mass spectrum (MS). 【Result 】About 1500 protein points were detected, and 66 different proteins were expressed at four different development stage of style in ‘Xinshiji’. Self and non-self pollen had triggered different effects on style at proteomic level. There was one protein expressed special in cross-pollinated style, and four up-expressed and twenty-nine down-expressed in cross-pollinated styl