4种纤维头对草莓香气成分的萃取效果.pdfVIP

中国农业科学 2010,43(21):4473-4481 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2010.21.017 4种纤维头对草莓香气成分的萃取效果 付 蕾，刘正生，孙鑫洋，朱树华 （山东农业大学化学与材料科学学院，山东泰安 271018 ） 摘要：【目的】研究不同固相微萃取纤维头对草莓香气成分的萃取效果和质谱数据的自动鉴定方法，为草莓品 质育种及其深加工提供一种简单、准确的研究方法。【方法】采用固相微萃取-气相色谱/质谱-自动质谱解卷积鉴定 系统检测成熟草莓果实中的香气成分。【结果】在成熟草莓果实中共检测到97种香气成分，主要成分为酯类、醇类、 酮类和萜烯类化合物，分别占已检出香气成分总数的 42.3%、13.4%、10.3%和 6.2%。草莓的特征香气成分至少有 8 种，根据贡献大小，依次为丁酸乙酯、己酸乙酯、沉香醇、呋喃酮、丁酸甲酯、己酸甲酯、2-庚酮和橙花叔醇。50/30 µm DVB/CAR/PDMS纤维头的灵敏度最高、检出的物质最多，其余依次是100 µm PDMS纤维头、85 µm PA和7 µm PDMS。 4 种固相微萃取纤维头对酯类物质均有较好的萃取效果，50/30 µm DVB/CAR/PDMS 纤维头对酮类、萜烯类、醇类 和醛类物质的萃取效果明显高于其它纤维头。【结论】50/30 µm DVB /CAR /PDMS纤维头较适用于草莓香气成分的 萃取。自动质谱解卷积鉴定系统处理鉴定质谱数据具有灵敏度高、准确性好、速度快的特点，值得推广应用。 关键词：固相微萃取；草莓；气-质联用；香气成分；自动质谱解卷积鉴定系统 Study of Four Coating Materials of SPME Fiber on Extraction of Aroma Compounds in Strawberry FU Lei, LIU Zheng-sheng, SUN Xin-yang, ZHU Shu-hua (College of Chemistry and Material Science ，Shandong Agricultural University, Tai’an 271018, Shandong) Abstract: 【Objective 】 In this paper the extraction of strawberry aroma compounds with four coating materials of solid-phase micro-extraction (SPME) fiber and the automatic identification method for gas chromatography/mass spectrometry (GC/MS) data were researched. The aim of this experiment was to summarize a simple, accurate method for quality breeding and processing of strawberry. 【Method 】 The aroma compounds in strawberry were detected by SPME-GC/MS- the automatic mass spectral deconvolution and identification system (AMDIS). 【Result 】 A total of 97 compounds were identified from the sample extracts. The major constituents were esters, alcohols, ketones and terpenes, with the