蝉拟青霉杀虫活性成分的分离.pdfVIP

中国农业科学 2007,40(9):1952-1958 Scientia Agricultura Sinica 蝉拟青霉杀虫活性成分的分离 柴一秋，金轶伟，刘又高，厉晓腊，陈官菊，王根锷 （浙江省亚热带作物研究所，温州 325005 ） 摘要：【目的】采用化学分离分析和生物检测相结合的方法，首次从蝉拟青霉[Paecilomyces cicadae （Miq.） Samson]子实体提取物中分离出对小菜蛾[Plitella xylostella （Lepidoptera：Plutellidae）]具有杀虫活性的 化合物。【方法】在活性检测引导下，蝉拟青霉子实体 40%乙醇提取物经二氯甲烷、乙酸乙酯、正丁醇溶剂依次 萃取（剩余溶液为水溶组分），再经过大孔树脂、HPLC和 Sephedex LH-20分离纯化得到杀虫活性化合物。【结果】 分离纯化到一个白色粉末状化合物，浓度10 mg·ml-1 ，24、48和72 h时校正死亡率分别为（95.1±1.8）%、（97.7 ±1.6）%和（99.1±1.7）%。【结论】该化合物具有较好的杀小菜蛾活性，对酸稳定，对胃蛋白酶不敏感，并具有 茚三酮不显色等其它理化性质。 关键词：蝉拟青霉；子实体提取物；小菜蛾；杀虫活性 Separation of Insecticidal Ingredient of Paecilomyces cicadae (Miquel) Samson CHAI Yi-qiu, JIN Yi-wei, LIU You-gao, LI Xiao-la, CHEN Guan-ju, WANG Gen-e (Zhejiang Institute of Subtropical Crops, Wenzhou 325005) Abstract: 【Objective 】Insecticidal active substance of the fruiting body of Paecilomyces Cicadae (Miquel) Samson that can kill the larvae of Plutella xylostella was isolated and purified by combined separation with biology experiments for the first time. 【Method 】 By means of bioactive guided isolation of 40% ethanol-H20, dichloromethane, ethyl acetate, n-butyl ethanol, macroporous adsorption resin column, high performance liquid chromatography and sephadex A-20 column, the insecticidal active compound was purified. 【Result 】 A white powder compound was obtained. The compound was active against the third instar larvae of P. xylostella L. in 24, 48 and 72 h at the concentration of 10.0 mg·ml-1 with the corrective mortality of (95.1±1.8)%, (97.7±1.6)% and (99.1±1.7)%, respectively. 【Conclusion 】The compound was strongly sensitive to the larvae. It was stable to acid, and was not sensitive to pepsin. Other physical and chemical characteristics were reporte