河西绒山羊次级毛囊超微结构的周期性变化.pdfVIP

中国农业科学 20 12,45(13):2779-2786 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2012.13.024 河西绒山羊次级毛囊超微结构的周期性变化 贺延玉, 罗玉柱, 程李香, 王继卿, 刘 秀 （甘肃农业大学甘肃省草食动物生物技术重点实验室/研究测试中心，兰州 730070 ） 摘要：【目的】研究成年绒山羊次级毛囊组织超微结构的周期性变化过程，为研究绒山羊绒毛生长的分子调 控机理奠定形态学基础。【方法】活体采集的皮肤样品，经 3%的戊二醛前固定和锇酸的后固定，通过上行酒精梯 度脱水后进行包埋。制作河西绒山羊一年周期内每个月的皮肤超薄切片，经铀和铅双染色后在透射电镜下进行超 微结构的观察、拍照和分析。【结果】河西绒山羊次级毛囊组织的超微结构随着毛生长周期呈周期性变化，可分为 5 个不同的时期，即1—2 月为休止期、3—4 月为生长前期、5—8 月为生长期、9—10 月为退行前期、11—12 月 为退行期。【结论】阐述了河西绒山羊次级毛囊在一个完整的生长循环过程中所处的5 个不同的时期，为绒山羊绒 毛相关领域的研究奠定超微结构方面的基础。 关键词：绒山羊; 次级毛囊; 超微结构 Identification of the Secondary Follicle Cycling of Hexi Cashmere Goat HE Yan-yu, LUO Yu-zhu, CHENG Li-xiang, WANG Ji-qing, LIU Xiu (Gansu Key Laboratory of Herbivorous Animal Biotechnology/Instrumental Research and Analysis Center, Gansu Agricultural University, Lanzhou 730070) Abstract: 【Objective 】An experiment was conducted to identify the periodic change of ultrastructures of secondary follicle characteristics in the whole year, reveal the molecule regulation of growth of cashmere goat. 【Method 】 A total of six cashmere goats of one year old were used in this experiment. The ultrathin slices of skin were made each month in the whole year, observed and photographed under transmission electron microscope after staining. 【Result 】Following the development of down fiber, the ultrastructures of secondary follicle of Hexi cashmere goat showed a periodic change within a year. There were five different periods during the down fiber cycle. It was observed that the stages of telogen, pregrowing, anagen, precatagen and catagen were in January and February, March and April, May to August, September and October and November and De