咪唑乙烟酸抑制大豆根瘤固氮酶活性的机理研究.pdfVIP

中国农业科学 2013,46(19):4173-4178 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2013.19.023 咪唑乙烟酸抑制大豆根瘤固氮酶活性的机理研究 李 慧，丁 伟，姜俊凤，王怀彪 （东北农业大学农学院植物保护系，哈尔滨150030） 摘要：【目的】探索咪唑乙烟酸抑制大豆根瘤固氮酶活性的关键机制，为解决咪唑乙烟酸长期大量应用对根 瘤固氮的毒害提供依据。【方法】采用田间试验方法，研究咪唑乙烟酸茎叶处理对根瘤固氮酶活性的影响与根瘤豆 血红蛋白含量、根瘤类菌体和细胞浆中氨含量的关系。【结果】咪唑乙烟酸施用后21 d 内，固氮酶活性受到显著 2 抑制。112.5 和225.0 g a.i./hm 施药量下，对豆血红蛋白含量的抑制峰值分别出现在第7、14 天，抑制率分别 为30.52%和35.41%，抑制作用分别在第21、28 天得到解除。根瘤类菌体中氨含量抑制峰值均出现在第14 天，抑 制率分别为35.33%和48.96%，第28 天氨含量均恢复正常。细胞浆中氨含量显著增高，且分别在第 21、28 天恢 复正常。【结论】咪唑乙烟酸茎叶处理后，豆血红蛋白含量降低是导致固氮酶活性降低的1 个重要原因，同时细胞 浆中氨积累也造成了对固氮酶活性的反馈抑制。 关键词：咪唑乙烟酸；大豆；根瘤固氮酶；豆血红蛋白；氨含量 Research on the Inhibitory Mechanism of Imazethapyr to Nodule Nitrogenase Activity in Soybean LI Hui, DING Wei, JIANG Jun-feng, WANG Huai-biao (Department of Plant Protection, Agronomy Coll ege, Northeast Agricultural University, Harbin 150030) Abstract: 【Objective 】The objective of this study is to discover the key inhibitory mechanism of imazethapyr to nodule nitrogenase activity in soybean for providing methods to resolve the toxity on nodule nitrogen fixation after imazethapyr being over used. 【Method 】A field experiment was conducted to determine the relationship between inhibited nodule nitrogenase activity and leghemoglobin content, ammonia content in nodule rhizobium and in nodule cytoplasm after imazethapyr pre-emergence used. 【Result 】Nodule nitrogenase activity was inhibited more pronounced during 21 days after treatment (DAT). One the 7 and 14 DAT, the inhibited ratio of leghemoglobin content peaked 30.52% and 35.41%, respectively at 112.5 and 25.0 g a.i./hm2 imazethapyr a