苦马豆素-BSA免疫山羊的血清相关酶评价.pdfVIP

中国农业科学 2006,39(11):2384-2389 Scientia Agricultura Sinica 苦马豆素-BSA免疫山羊的血清相关酶评价 童德文，董 强，赵宝玉，刘文明，李 立，赵 俊 （西北农林科技大学动物科技学院，杨凌 712100 ） 摘要：【目的】通过评价 SW-BSA 免疫山羊的血清相关酶，探索 SW-BSA 免疫接种后对动物机体组织器官的保 护作用。【方法】将 24 只山羊随机分为免疫对照组、免疫攻毒组和攻毒组，其中免疫对照组和免疫攻毒组接种 SW-BSA，免疫攻毒组和攻毒组拌料饲喂10 g/kg BW/d甘肃棘豆草粉攻毒。【结果】血清GOT、GPT、LDH、AKP、BUN、 AMA和抗体效价的检测结果表明，免疫攻毒组山羊较攻毒组山羊LDH活性升高延缓28 d，AKP活性升高延缓14 d， AMA活性降低延缓21 d，BUN 活性升高延缓14 d，GOT活性升高延缓28 d。攻毒后免疫攻毒组山羊抗SW抗体水平 降低，但在攻毒的第 21 天有一反弹，之后逐渐下降。【结论】这些酶活性延缓变化和抗体水平变化，说明在甘肃 棘豆攻毒的前30 d内，SW-BSA能够有效地延缓SW对山羊肝脏、心脏和肾脏等组织器官的损伤。 关键词：SW-BSA；血清酶；甘肃棘豆；山羊 Evaluation on Related Serum Enzyme of SW-BSA Immune Goats TONG De-wen, DONG Qiang, ZHAO Bao-yu, LIU Wen-ming, LI Li, ZHAO Jun (College of Animal Science and Technology, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100) Abstract ：【Objective 】The study evaluated serum enzyme of SW-BSA immune goats to probe the protection of the animal’s tissue and organ protection after vaccinating with SW-BSA. 【Method 】 24 goats were randomly separated into 4 groups. Goats were placed in an immune control, and goats placed in the immune poisoning groups were vaccinated with SW-BSA. Goats in immune poisoning and poisoning groups were fed 10 g/kg BW/d power of O. kansuensis Bunge. 【Result 】 The changes of serum GOT, GPT, LDH, AKP, BUN, AMA and antibody against SW showed that the increasing level of LDH and GOT had been delayed 28 days. The increasing level of AKP and BUN had been delayed 14 days, the decreasing level of AMA had been delayed 21 days. This was in immune poisoning goats, as compared to the poisoning goats. The antibody level against SW decreased. However, there was a rebound at day 21, and then it decreased gradually. 【Conclusion 】These result