利用RNAi技术防治水稻条纹叶枯病.pdfVIP

中国农业科学 2011,44(13):2683-2691 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2011.13.006 利用RNAi 技术防治水稻条纹叶枯病 张 燕，伏红伟，王 爽，梁国华，杨益众 （扬州大学园艺与植物保护学院，江苏扬州 225009 ） 摘要：【目的】控制水稻条纹叶枯病的发生与流行，改变当前水稻一旦染毒就无药可治的现状。【方法】采用 浸种与喷雾技术使生物疫苗进入稻株体内，通过稻株体内RSV 带毒情况和疫苗残留情况室内检测以及水稻条纹叶 枯病发病率的田间调查，确定生物疫苗对RSV 表达以及条纹叶枯病的控制效果。【结果】在疫苗中添加1.0 ‰的B 渗透剂是浸种的最适浓度，添加 5.0‰的A 渗透剂是喷雾的最适浓度；稻株体内疫苗检出率的比例与RSV 的降解 呈现一致性；浸种处理后疫苗在稻株体内能维持30 d 以上，喷雾处理后的残留期为10—20 d。2 种处理方式对条 纹叶枯病的控制效果均超过了70%。【结论】生物疫苗可以作为一种新型的生物农药用于水稻条纹叶枯病的田间防 治。 关键词：RNAi 技术；基因疫苗；水稻条纹病毒；浸种与喷雾；控制效果 Control of Rice Stripe Disease Using RNAi Technology ZHANG Yan, FU Hong-wei, WANG Shuang, LIANG Guo-hua, YANG Yi-zhong (School of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009，Jiangsu) Abstract: 【Objective 】The objective of this study is to control the incidence and prevalence of rice stripe disease, change the situation of that there is no specific pesticide to inhibit the occurrence of rice stripe disease once rice is infected with RSV. 【Method 】 Seed soaking and spraying technologies were used to make biological vaccines absorbed by rice plants. Both the detection of RSV-carrying rate and vaccine residue in rice plant and field survey on occurrence rate of rice stripe disease were conducted to determine vaccine control efficiency to the disease. 【Result 】 Addition of 1.0‰ B penetrant in the vaccine was the optimal concentration for seed soaking and 5.0‰ A penetrant was the optimal concentration for spraying. The detection rate of vaccine was consistent with the degeneration of RSV. The residue time of vaccine in rice plant was more than 30 d after seed soaking and 10-20 d after spraying. The