利用弱毒株交叉保护技术防治甜橙茎陷点型衰退病.pdfVIP

中国农业科学 2008,41(12):4085-4091 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2008.12.019 利用弱毒株交叉保护技术防治甜橙茎陷点型衰退病 周 彦，周常勇，李中安，王雪峰，刘科宏 （西南大学柑桔研究所/ 中国农业科学院柑桔研究所，重庆 400712 ） 摘要：【目的】获得对中国甜橙茎陷点型衰退病具有防治作用的柑桔衰退病毒(Citrus tristeza virus，CTV) 弱毒株。【方法】对 7 个国内外 CTV 弱毒分离株进行褐色桔蚜接种强毒株的拮抗试验，并运用p25/HinfⅠRFLP 组 群分析、特异性 RT-PCR 分析和寄主植株症状观察的方法对拮抗试验效果进行检测。【结果】本研究中所用 7 个弱 毒株对甜橙茎陷点型衰退病均有一定的防治作用，其中弱毒株 CT9、CT17 和 CT77 对茎陷点型强毒株 CT14 的拮抗 效果最为明显，分别为70%，50%和40%。分子检测与生物学检测的结果相一致。【结论】初步筛选出的具有拮抗作 用的CTV弱毒株CT9、CT17 和CT77具有应用于交叉保护的潜力，可供进一步田间区试研究。 关键词：柑桔衰退病毒；交叉保护；防效检测 Mild Strains Cross Protection Against Stem-Pitting Tristeza of Sweet Orange ZHOU Yan, ZHOU Chang-yong, LI Zhong-an, WANG Xue-feng, LIU Ke-hong (Citrus Research Institute, Southwest University/Citrus Research Institute, Chinese Academy of Agricultural Sciences, Chongqing 400712) Abstract: 【Objective 】This study was conducted to obtain the protective mild isolates of Citrus tristeza virus (CTV) to protect sweet orange plants against super-infection of the severe stem-pitting CTV isolates. 【Method 】Sweet orange seedlings were graft-inoculated with seven mild CTV isolates and challenge-inoculated with severe stem-pitting isolates born by brown citrus aphids (Toxoptera citricida Kirkaldy). The effectiveness of the cross protection was evaluated by p25/Hin f ⅠRFLP groups assay, special RT-PCR assay and stem-pitting examination. 【Result 】Seven CTV mild isolates applied in this study were all experimentally proven to have somehow protective effectiveness against severe stem-pitting CTV isolates, of which mild isolates of CTV CT9, CT17 and CT77 had more significant effectiveness than the others. 【Conclusion 】The mild CTV isolates