荔枝果皮花青苷合成与相关酶的关系研究.pdfVIP

中国农业科学 2004,37(12):2028-2032 Scientia Agricultura Sinica 荔枝果皮花青苷合成与相关酶的关系研究 王惠聪 黄旭明 胡桂兵 黄辉白 华南农业大学园艺学院南方果树生理实验室 广州 510642 摘要 以 妃子笑 荔枝为试材 研究果实成熟过程中 果皮中花青苷含量及其生物合成相关的苯丙氨酸 解氨酶 PAL 查儿酮异构酶 CHI 二氢黄酮醇还原酶 DFR 和类黄酮糖基转移酶 UFGT 的酶活性变 化 同时研究套袋和生长调节剂对花青苷合成和相关酶活性的影响 结果表明 在4 种酶中只有UFGT 活性与 荔枝果皮花青苷合成关系密切 UFGT 活性的变化与花青苷含量的变化趋势吻合 随着 妃子笑 果皮中UFGT 活性的增加 花青苷含量上升 套袋处理抑制UFGT 活性的同时也抑制花青苷的合成 除袋后UFGT 活性和花 青苷含量都迅速增加 6-BA 处理抑制UFGT 酶活性的同时也抑制花青苷合成 ABA 和茉莉酸处理提高了UFGT 酶活性的同时也促进了花青苷的合成 关键词 荔枝 果皮 花青苷 PAL CHI DFR UFGT Studies on the Relationship Between Anthocyanin Biosynthesis and Related Enzymes in Litchi Pericarp WANG Hui-cong, HUANG Xu-ming, HU Gui-bing, HUANG Hui-bai (Physiology Laboratory for Southern Fruits, College of Horticulture, South China Agricultural University, Guangzhou 510642) Abstract: Changes in the level of anthocyanin and the activities of enzymes of anthocyanin biosynthesis including plenylalanine ammonia lyase (PAL), chalcone ismoerase (CHI), dihydroflavonol reductase (DFR) and UDP glucose-flavonoid -3- o-glycosyltranfe-rase (UFGT) were studied in the pericarp of litchi cv. Feizixiao. Bagging and growth regulator dipping treatments were conducted to manipulate anthocyanin synthesis and the activities of related enzymes. The results demonstrated that among these enzymes UFGT was closely related to the synthesis of anthocyanin and probably was the limiting factor regulating anthocyanin biosynthesis in litchi pericarp. The activity of UFGT was in parallel with the changes of anthocyanin concentration. Bagging treatment inhibited the activity of UFGT as well as anthocyanin synthesis, whereas deba