利用单片段代换系定位水稻抽穗期QTL.pdfVIP

中国农业科学 2005,38(8):1505-1513 Scientia Agricultura Sinica 利用单片段代换系定位水稻抽穗期 QTL 何风华，席章营，曾瑞珍，Akshay Talukdar，张桂权 （华南农业大学植物分子育种广东省重点实验室，广州 510642 ） 摘要：抽穗期是水稻品种的重要农艺性状之一，对抽穗期QTL 进行定位并研究其遗传效应在水稻育种中是至 关重要的。本研究利用以6 个水稻品种为供体的52 个单片段代换系为试验材料，通过t 测验比较单片段代换系与 受体亲本华粳籼74 之间抽穗期的差异，对代换片段上的抽穗期QTL 进行了鉴定。以P ≤0.001 为阈值共鉴定出20 个抽穗期QTL ，这些QTL 分布于水稻的 10 条染色体。QTL 加性效应值为–5.9 ～1.1，加性效应百分率为–7.4% ～ 1.4%。有8 个QTL 被定位在小于 10.0 cM 的区段内。利用1 个单片段代换系与华粳籼74 杂交发展的F2 群体对 qHD-3-1 进行了定位。在作图群体中，早抽穗和迟抽穗植株数符合 3:1 的分离比，早抽穗表现为显性。利用微卫 星标记将qHD-3-1 定位于3 号染色体短臂，PSM304 和RM569 分别位于其两侧，遗传距离分别为2.4 cM 和5.1 cM。 关键词：水稻；单片段代换系；抽穗期；QTL；基因定位 Mapping of Heading Date QTLs in Rice (Oryza sativa L.) Using Single Segment Substitution Lines HE Feng-hua, XI Zhang-ying, ZENG Rui-zhen, Akshay Talukdar, ZHANG Gui-quan (Guangdong Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou 510642) Abstract: Heading date (HD) is an important agronomic trait in rice. Appropriate heading date is a prerequisite for attaining the desired yield level in rice breeding programs. It is very important for rice improvement to map heading date quantitative trait loci (QTLs). Single segment substitution lines (SSSLs) containing only one segment of donor chromosome are useful tools for precise mapping of QTLs and for the evaluation of gene action. In this research, the QTLs for heading date on the substituted segments of 52 rice SSSLs derived from 6 advanced backcrosses were identified through t-test for means comparisons between SSSLs and the recipient parent Huajingxian 74. QTLs were detected with a significance of P ≤0.001. A total of 20 QTLs were identified on 10 rice chromosomes with the exception of chromosomes 5 and 6. The QTL additive effect ranged from –