利用表型和SSR标记分析河南省玉米地方品种的遗传多样性.pdfVIP

中国农业科学 2009,42(4):1136-1144 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2009.04.002 利用表型和SSR标记分析河南省玉米地方品种的遗传多样性 王利锋，李会勇，唐保军，程泽强，王振华，铁双贵 （河南省农业科学院粮食作物研究所，郑州 450002 ） 摘要：【目的】分析河南省玉米地方品种的遗传多样性，为进一步改良利用提供合理方案。【方法】利用表型 和 SSR 标记基因型，对 88 份有代表性的地方品种进行遗传多样性分析。【结果】这些地方品种农艺性状表现出较 大差异，表型聚类将其划分为7个类群，大部分品种聚在一个类群内，但仍有部分品种独立成群。SSR分析表明， 每对引物可以稳定检测到 2～10 个等位基因，40 对 SSR 引物共检测到 198 个等位基因，平均每个位点 4.95 个等 位基因，遗传相似系数为 0.63～0.89。SSR 标记聚类分析同样将此材料划分为 7 个类群，大部分品种仍主要集中 在一个主群内。Mental 测验结果表明，表型同基因型距离矩阵间存在极显著正相关(r=0.78，P=0.01)。【结论】 河南省玉米地方品种来源较单一，遗传多样性较差，仅少部分品种较为独立，可有针对性地保存和改良利用。 关键词：玉米；河南省；地方品种；SSR；遗传多样性 Genetic Diversity Analysis of Henan Maize Landrace by Phenotype and Simple Sequence Repeat (SSR) Markers WANG Li-feng, LI Hui-yong, TANG Bao-jun, CHENG Ze-qiang, WANG Zhen-hua, TIE Shuang-gui (Cereal Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002) Abstract: 【Objective 】 To provide a reasonable further improved program, genetic diversity of maize landrace in Henan was analyzed. 【Method 】 Genetic diversity of 88 maize landraces was analyzed by phenotypes and SSR genotype in this research. 【Result 】 These maize germplasms varied significantly in all of the measured morphological traits. Phenotype cluster analysis showed the 88 accessions were divided into seven groups, and most of the landraces were clustered into one group. However, a few varieties could be clustered into another single group. 2-10 polymorphic fragments were amplified stably by each SSR marker. Total 198 alleles were detected at 40 loci and the average number of alleles was 4.95. Genetic dissimilarity coefficients ranged from 0.63 to 0.89. These maize germplasms were also could be clustered i