水杨酸对厚皮甜瓜采后病害及苯丙烷代谢的影响.pdfVIP

中国农业科学 2012,45(3):584-589 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2012.03.022 水杨酸对厚皮甜瓜采后病害及苯丙烷代谢的影响 范存斐，毕 阳，王云飞，任亚琳，杨志敏，王 毅 （甘肃农业大学食品科学与工程学院，兰州730070 ） 摘要：【目的】探讨采后水杨酸处理对厚皮甜瓜果实采后病害的抑制效果和苯丙烷代谢的增强作用。【方法】 以‘玉金香’厚皮甜瓜为试材，用4 mmol·L-1 水杨酸采后常温浸泡10 min，测定处理对粉红单端孢（Trichothecium roseum）损伤接种果实病斑直径和自然发病率的抑制，以及对果实苯丙烷代谢关键酶活性和产物积累的影响。【结 果】水杨酸处理不仅明显降低了果实损伤接种T. roseum 的病斑直径，而且还显著减轻了常温贮藏期间果实的自 然发病率。水杨酸处理明显提高了苯丙氨酸解氨酶（PAL）、4-香豆酰-辅酶A 连接酶（4CL）和肉桂酸羟化酶（C4H） 的活性，处理后6 d，其C4H 和4CL 活性分别比对照高1.0 和2.8 倍，10 d 时PAL 活性为对照的1.6 倍。此外， 处理还显著提高了果实的总酚以及木质素含量，处理后4 d 和10 d 果实木质素和总酚含量分别高出对照30.6%和 30.0%，但处理对类黄酮含量影响不大。【结论】采后水杨酸处理能够通过诱导厚皮甜瓜的苯丙烷代谢增强果实对 采后病害的抗性。 关键词：厚皮甜瓜；水杨酸；苯丙烷途径；采后病害 Effect of Salicylic Acid Dipping on Postharvest Diseases and Phenylpropanoid Pathway in Muskmelon Fruits FAN Cun-fei, BI Yang, WANG Yun-fei, REN Ya-lin, YANG Zhi-min, WANG Yi (College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070) Abstract: 【Objective 】The effects of salicylic acid (SA) dipping on postharvest disease resistance and phenylpropanoid pathway in muskmelon fruit was investigated in this paper. 【Method 】Muskmelons (Cucumis melo L. cv.Yujinxiang) were dipped at 4 mmol ·L-1 of SA for 10 min. The inhibition effect of SA dipping on the lesion diameter of fruits inoculated with Trichothecium roseum and natural incidence of melons were investigated during storage at room temperature. The key enzyme activities and substances accumulation of phenylpropanoid pathway in fruits were also detected. 【Result 】 SA significantly decreased the lesion diameter of fruits inoculated with T. roseum, and the natural incidence of fruits during storage at roo