甜菜夜蛾抗茚虫威品系的生物适合度及抗性遗传方式.pdfVIP

中国农业科学 2011,44(18):3741-3749 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2011.18.005 甜菜夜蛾抗茚虫威品系的生物适合度及抗性遗传方式 王 伟，高 明，邓兆荣，李秀环，慕 卫 （山东农业大学植物保护学院，山东泰安 271018 ） 摘要：【目的】测定甜菜夜蛾（Spodoptera exigua）抗茚虫威品系对8 种常用杀虫剂的交互抗性、生物适合 度及抗性遗传方式。【方法】生测方法确定交互抗性程度，组建种群生命表评价抗性品系的生物适合度，LD-P线与 统计分析相结合研究抗性遗传方式。【结果】对茚虫威产生134 倍抗性的甜菜夜蛾品系（RR-indox）除对甲维盐表 现中等水平的交互抗性（12.59 倍）外，对其它 7 种药剂均没有明显交互抗性。抗性品系相对敏感品系的生物适 合度为0.44，表现出一定程度的生长发育和繁殖上的不利性。杂交后代（F ，R♀×S ♂；F′ ，S♀×R ♂）的显性 1 1 度分别为0.54 和0.74，甜菜夜蛾对茚虫威的抗性为常染色体多基因，不完全显性遗传。【结论】茚虫威防治甜菜 夜蛾应注意与无交互抗性药剂轮换使用，以延缓抗性的产生。 关键词：茚虫威；甜菜夜蛾；交互抗性；生物适合度；抗性遗传 Biologic Relative Fitness and Genetics of Indoxacarb Resistance in Spodoptera exigua (Hübner) WANG Wei, GAO Ming, DENG Zhao-rong, LI Xiu-huan, MU Wei (College of Plant Protection, Shandong Agricultural University, Taian 271018, Shandong) Abstract: 【Objective 】 The objective of this study is to determine the cross-resistance, relative fitness and resistance inheritance of the selected strain of Spodoptera exigua (Hübner) to indoxacarb in the laboratory. 【Method 】 The leaf-dip bioassay method was used for confirming the degrees of cross-resistance. The relative fitness was evaluated by constituting the life tables of different strains. The inheritance mode of resistance to indoxcarb in S. exigua was evaluated by using log dosage-probit mortality curves and statistical methods. 【Result 】 The 134-fold resistant strain (RR-indox) showed moderate level cross-resistance to emamectin benzoate (12.59-fold) and little or no cross-resistance to the other 7 insecticides tested. The RR-indox strain had a fitness value of 0.44 relati