铜对苋菜幼苗光合参数和活性氧代谢的影响.pdfVIP

中国农业科学 2008,41(5):1317-1325 Scientia Agricultura Sinica 铜对苋菜幼苗光合参数和活性氧代谢的影响 柯世省 （台州学院生命科学学院，浙江临海 317000 ） 摘要：【目的】明确铜毒影响苋菜（Amaranthus tricolor ）幼苗光合作用和活性氧代谢的生理机制。【方法】 采用土培试验测定了不同水平铜处理下苋菜幼苗生长、光合作用、矿质元素吸收和活性氧代谢的相关指标。【结果】 2.0～4.0 mmol·kg-1 铜处理下植株干重降至无铜处理（对照）的91%～73%，净光合速率和气孔导度均明显减小，但 胞间CO2 浓度并没发生显著变化，最大光化学效率的变化比实际量子效率小得多，这暗示着光合作用暗反应对ATP 和NADH需求减少使光化学下调。此外，叶片光合色素、水分和矿质元素Fe、K和Mg 含量均明显降低，Cu含量明 显升高。2.0～4.0 mmol·kg-1 铜处理显著增加叶片超氧化物歧化酶、过氧化物酶活性和脯氨酸含量，降低过氧化氢 酶、抗坏血酸过氧化物酶活性及类胡萝卜素、还原型谷胱甘肽和抗坏血酸含量，并伴随丙二醛含量和细胞电解质 渗漏率增加。【结论】2.0 mmol·kg-1 以上铜处理引起苋菜活性氧代谢失衡，明显降低净光合速率和实际量子效率， 加剧过氧化作用，导致植株生长严重受阻。 关键词：苋菜；铜毒；光合作用；活性氧代谢 Effects of Different Copper Levels on Photosynthetic Parameters and Active Oxygen Metabolism of Amaranthus tricolor Seedlings KE Shi-sheng (School of Life Sciences, Taizhou University, Linhai 317000, Zhejiang) Abstract: 【Objective 】The objective of this study was to gain better insight into the physiological mechanisms of copper (Cu) on photosynthesis and active oxygen metabolism in three-coloured amaranth plant (Amaranthus tricolor). 【Method 】 Three-coloured amaranth seedlings were subjected to different Cu levels in soils during the whole experimental period. The parameters of growth, photosynthesis, mineral elements contents and active oxygen metabolism were investigated by using plant physiological methods. 【Result 】2.0-4.0 mmol Cu·kg-1 treatment decreased the whole plant dry mass to 91%-73%. Net photosynthetic rate (Pn) and stomalal conductance (Gs) in the third leaves of three-coloured amaranth seedlings treated with 2.0-4.0 mmol Cu·kg-1 were similarly reduced. None of the investigated Cu levels decreas