小麦生理型雄性不育花药绒毡层和孢粉素变化与RAFTIN1表达的关系.pdfVIP

中国农业科学 2011,44(19):3937-3944 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2011.19.003 小麦生理型雄性不育花药绒毡层和孢粉素变化 与RAFTIN1 表达的关系 盛 英, 张改生, 李亚鑫, 张龙雨, 王书平, 赵新亮, 王亮明, 宋瑜龙 （西北农林科技大学农学院/ 陕西省作物杂种优势研究与利用重点实验室/小麦育种教育部工程研究中心，陕西杨凌 712100 ） 摘要：【目的】研究小麦生理型雄性不育花药绒毡层变化、孢粉素累积与RAFTIN1 表达间的关系，为揭示小 麦生理型雄性不育的机理奠定基础。【方法】以杀雄剂SQ-1 诱导的生理型雄性不育系、质核互作遗传型雄性不育 系，正常可育近等基因系为试材，通过石蜡切片、细胞荧光染色和荧光定量PCR 技术，研究小孢子不同发育时期 花药绒毡层的形态变化、孢粉素的累积及RAFTIN1 的表达。【结果】单核期生理型不育系花药绒毡层提前降解，分 泌孢粉素的含量降低，RAFTIN1 提前高表达，使大量孢粉素转运至花粉壁层；二核期和三核期，生理型不育系绒 毡层完全退化，停止分泌孢粉素，RAFTIN1 呈现明显的下调表达模式。【结论】杀雄剂 SQ-1 诱导的小麦生理型雄 性不育其败育机理与绒毡层的提前降解和定向转运孢粉素的RAFTIN1 表达高低直接相关。 关键词：小麦(Triticum aestivum.L.）；杀雄剂SQ-1；绒毡层；孢粉素；RAFTIN1；实时荧光定量PCR The Relationship on Anther Tapetum, Sporopollenin and Expression of RAFTIN1 in Physiological Male Sterile Wheat SHENG Ying, ZHANG Gai-sheng, LI Ya-xin, ZHANG Long-yu, WANG Shu-ping, ZHAO Xin-liang, WANG Liang-ming, SONG Yu-long (College of Agronomy, Northwest Agriculture and Forestry University /Key Laboratory of Crop Heterosis of Shaanxi Province/Wheat Breeding Engineering Research Center, Ministry of Education, Yangling 712100, Shaanxi) Abstract: 【Objective 】 To pave the way for revealing the mechanism of physiological male sterility in wheat, the relevance among expression of RAFTIN1 , anther tapetum and sporopollenin were studied. 【Method 】Using physiologically male sterility induced with chemical hybridizing agent SQ-1, genetically male sterility and corresponding normal lines of complete fertility as experimental materials in wheat, morphological changes of anther tapetum from different developmental stages of microspore, sporopollenin accumulatio