中国葡萄灰霉病菌对嘧霉胺的抗药性检测.pdfVIP

中国农业科学 2013,46 6 : 1208-1212 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2013.06.014 中国葡萄灰霉病菌对嘧霉胺的抗药性检测 1 1 1 2 1 张 玮 ，乔广行 ，黄金宝 ，王忠跃 ，李兴红 1 2 （北京市农林科学院植物保护环境保护研究所，北京 100097； 中国农业科学院植物保护研究所，北京 100193） 摘要：【目的】检测中国葡萄灰霉病菌对苯胺基嘧啶类杀菌剂嘧霉胺的抗药性，明确中国不同葡萄产区灰霉 病菌对嘧霉胺的抗药性及抗性频率，为葡萄灰霉病的药剂防治提供理论基础。 【方法】从中国葡萄主产区采集、 分离纯化 104 个葡萄灰霉病菌（Botrytis cinerea Pers.）的单孢菌株，采用菌丝生长速率法测定其对嘧霉胺的 抗药性。 【结果】中国葡萄灰霉病菌对嘧霉胺的抗性频率为 22.22%—62.5%，且以高抗和中抗菌株为主，其中高 抗菌株频率达44.23%；葡萄不同气候栽培区的灰霉病菌对嘧霉胺抗药性不同。【结论】中国葡萄灰霉病菌对嘧霉 胺的抗药性较为普遍，且存在交互抗性，据此，在葡萄灰霉病的防治中应限制嘧霉胺的使用次数，可与二甲酰亚 胺类、氨基甲酸酯类杀菌剂交替使用；引进防治葡萄灰霉病的新型杀菌剂或生物农药。 关键词：葡萄；灰霉病菌；嘧霉胺；抗药性 Evaluation on Resistance of Grape Gray Mold Pathogen Botrytis cinerea to Pyrimethanil in China 1 1 1 2 1 ZHANG Wei , QIAO Guang-hang , HUANG Jin-bao , WANG Zhong-yue , LI Xing-hong 1 （Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, B eijing 100097; 2Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193 Abstract : 【Objective 】The resistance to pyrimethanil of Botry tis cinerea isolated from diseased grape berries in China was studied. 【Method 】A total of 104 single conidial isolates of B. cinerea were isolated from diseased grape berries which were collected from vineyards located in 14 different cities or suburban counties in China. The method of mycelial growth inhibition was used to determine the resistance of B. cinerea to anilinopyrimidine fungicides pyrimethanil. 【Result 】The findings in the current work demonstrated that the resistance frequency of B. cinerea to pyrimethanil was 22.22% to 62.5%. Most of the resistant isolates showed middle resistant or high resistant phenotype, and the resistant frequency of high resistant isolates reached up to 44.23%. Resistance to pyrimethanil of B. cinerea differs in different viticulture climate zones. 【Conclusion 】Resistance to pyrimethanil of B. cinerea isolated from grape diseased samples in