PROTEIN PATTERN OF - SciELO.pdfVIP

Received: October 24, 2005 J. Venom. Anim. Toxins incl. Trop. Dis. Accepted: January 23, 2006 V.12, n.4, p.612-619, 2006. Abstract published online: April 11, 2006 Original paper. Full paper published online: November 30, 2006 ISSN 1678-9199. INTRASPECIFIC VARIATION IN PROTEIN PATTERN OF RED SCORPION (Mesobuthus tamulus, COCONSIS, POCOCK) VENOMS FROM WESTERN AND SOUTHERN INDIA BADHE R. V. (1), THOMAS A. B. (1), HARER S. L. (1), DESHPANDE A. D. (1), SALVI N. (2), WAGHMARE A. (2) (1) Department of Pharmaceutical Chemistry, Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune, Maharashtra, India; (2) Haffkine Biopharmaceutical Corporation Ltd, Pimpri, Pune, Maharashtra, India. ABSTRACT: Red scorpions Mesobuthus tamulus (Coconsis, Pocock) were obtained from different regions of West and South India (Ratnagiri, Chiplun and Ahmednagar from Maharashtra and Chennai from Tamil Nadu, respectively). Their venoms composition was analyzed using gel electrophoresis (SDS). All venom samples shared six bands of 170, 80, 60, 57, 43, and 38 kDa molecular weights. Bands of 115 kDa and 51.5 kDa were characteristic of venoms obtained from red scorpions of Chiplun region, and the 26kDa band was absent in scorpion venom from Tamil Nadu. The separated protein band patterns suggest that the venoms from Ratnagiri, Ahmednagar and Tamil Nadu had high similarities in their biochemical composition but differed from that of Chiplun region. These data were also supported by the Jacca