紫杉醇脂质体与培美曲塞在乳腺癌细胞SK-BR-3中放疗增敏作用的比较及其机制.pdfVIP

· 2256 · SK—BR一3 机 紫杉醇脂质体与培美曲塞在乳腺癌细胞 SK—BR一3中放疗增敏作用的 比较及其机制 姚 炬 ，李索妮 ，阮之平 ，史婷婷 ，南克俊。，姚 煜 EffectofradiosensitizationbetweenPaclitaxeliiposomeandPemetrexedandthemecha— nism YaoXuan，LiSuoni，RuanZhiping，ShiTingting，NanKejun，YaoYu DepartmentofGeneralSurgery，CentralHospitalofXian，ShaanxiXian710003，China；DepartmentofOncology，TumorHospitalof Shaanxi，Shaanxi．n，n710061，China；DepartmentofOncology，theFirstAffiliatedHospitalofXi~tnJiaotongUniversity，Shaanxi 缸n 710061，China． 【Abstract】 0bjective：TocomparetheeffectofradiosensitizationbetweenPaclitaxelliposomeandPemetrexedand exploretheirmechanism inbreastcancercelllineSK—BR 一3．M ethods：MTrassaywasusedtotesttheinhibitory effectofPaclitaxelliposomecombinedwithradiotherapyandPemetrexedcombinedwithradiotherapyinbreastcancer celllineSK —BR一3．Flowcytometryinstrumenttodetectthecellcycleandapoptosis．Westernblottodetectapopto— sisrelatedproteinsBcl一2，Baxandcasepase一3．Results：ThecellgrowthinhibitionofPaclitaxelliposomecombined withradiotherapyshowedmoresignificantthanPemetrexedcombinedwith radiotherapyandsingleradiotherapy，the differencewasstatisticallysignificant(P0．05)．ApoptosisafterthePaclitaxelliposomecombinationgroupshowed moresignificantthanradiotherapygroupandPemetrexed combinedgroup，thedifferencewasstatisticallysignificant (P0．05)．Combinationgroupsandradiotherapygroupcausedtheappearanceofsub—G1peak，andcombination groupsshowedmoresignificantthanradiotherapyrgoup，thedifferencewasstatisticallysignificant(P0．05)．Con- clusion：TheeffectofradiosensitizationisstrongerthanPemetrexedandthroughregulatingtheexpressionofapoptosis proteinsPaelitaxelliposomeachievedtheeffectofradiosensitization． K【eywords】breastcancer；Pemetrexed；Paclitaxelliposome；radiosensitization；cellcycle；apotosis