原创力文档a specific assay for quantification of human c4c by use of an anti-c4c monoclonal antibody：用一个anti-c4c单克隆抗体人c4c量化具体检测.pdfVIP
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a specific assay for quantification of human c4c by use of an anti-c4c monoclonal antibody：用一个anti-c4c单克隆抗体人c4c量化具体检测
Journal of Immunological Methods 405 (2014) 87 –96
Contents lists available at ScienceDirect
Journal of Immunological Methods
journal homepage: www.el /l oca te/ jim
Research paper
A specific assay for quantification of human C4c by use of an
anti-C4c monoclonal antibody
Katrine Pilely a, Mikkel-Ole Skjoedt b, Christian Nielsen c, Thomas Emil Andersen d,
Anne Louise Aabom e, Lars Vitved a, Claus Koch a, Karsten Skjødt a, Yaseelan Palarasah a,⁎
a Department of Cancer Infl ammation Research, Institute of Molecular Medicine, Faculty of Health Science, University of Southern Denmark, Odense, Denmark
b Laboratory of Molecular Medicine, Department of Clinical Immunology, Rigshospitalet, Faculty of Health Sciences, University Hospital of Copenhagen,
Copenhagen, Denmark
c Department of Clinical Immunology, Odense University Hospital, Odense, Denmark
d Research Unit of Clinical Microbiology, Institute of Clinical Research, University of Southern Denmark, Odense, Denmark
e Department of Dermatology and Allergy Centre, Odense University Hospital, Odense, Denmark
a r t i c l e i n f o a b s t r a c t
Article history: The increasing evidence of the implication of the complement system in the pathogenesis of
Received 25 October 2013 several diseases has emphasized the need for the development of specific and valid assays,
Received in revised form 9 January 2014 optimized for quantitative detection of complement activation in vivo. In the present study, we
Accepted 9 January 2014 have developed a mouse monoclonal antibody (mAb) that is able to detect fluid phase C4c
Available online 2
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