精子dna完整与宫腔内人工授精妊娠率关系的相关研究.pdf

间呈显著负相关 （P ＜0.01 ）；4.IUI 妊娠组的精子DFI （20.33% ）比非妊 娠组的精子DFI （30.84% ）低，差异具有显著统计学意义 （t=3.499, P ＜ 0.01 ）；5.妊娠组与非妊娠组的精液量无明显差异 （t=1.457, P ＞0.05 ）， 妊娠组的精子密度和精子活率比非妊娠组高 （P ＜0.05 ），妊娠组精子正 常形态率 （25.33% ）显著高于非妊娠组的精子正常形态率 （14.95% ） （t=5.939, P ＜0.01 ）。结论：PureSperm 密度梯度离心法可以从精子DNA 完整度、形态及活率等方面较大程度的优化精液质量，精子DFI 与精子密 度、活率及正常形态率之间呈明显负相关，是影响IUI 妊娠结局的重要因 素，因此精子DFI 可作为精子DNA 损伤程度不同的不育男性选择不同辅 助生殖方式的一个参考指标，使那些精子DNA 损伤严重的通过IUI 受孕 机会较小的病例直接求助其他ART ，而对于那些精子DNA 损伤较小的病 例可进行数次IUI 治疗周期，从而避免使其接受其他更为复杂，花费更 高、更具侵袭性的ART 。 关键词：精子DNA ；Pure Sperm 密度梯度离心法；吖啶橙试验；宫腔内 人工授精；妊娠率 2 The Study of the Relationship Between Sperm DNA Fragmentation Index and Intrauterine Insemination Pregnancy Rate Abstract:Objective: Sperm DNA is an important parameter of sperm quality, foreign studies have shown that sperm DNA have obvious lili relation with sperm morphology and function.Sperm DNA is an important facnotor that could cause infertility and miscarriage.This experimentdafang yidian has an exploration in the relationship between sperm DNAdafangni d xing damage and the intrauterine insemination pregnancy rateyida by using acrimeiou wen wo dine orange test(AOT) to refer different degree of sperm DNA damagbuyao guane to provide theoretical support for different infertile patientcanjia shenm s to choose different assisted reproductive technology.PureSperm densityzhongji gedou -gradient centrifugatiomeiyoukissing n is one of the most popular semen optimiyoudian xiang zation processing methods.Thiget outs study is to analyze the influence PureSpshuaijiao erm density-gradient centrifugation adating ct on sperm DNA and other sperm pyemanarameters by testing sperm DNA and otherboth parameters before and after sisa the optimization processing method. Methods: 46you semen samples were obtmeiyou yuansained from 36 male attending medi dong ical reproduction center of affi