3491-马唐草新月弯孢生物学特性.docVIP

马唐草新月弯孢生物学特性 及代谢产物活性测定 杨 叶1, 胡美姣2, 王兰英1 (1.海南大学环境与植物保护学院，海南儋州，571737;2. 中国热带农业科学院环境与植物保护研究所，海南儋州，571737) 摘要：对马唐草新月弯孢霉（Curvularia lunata (Wakker) Boedijn）菌株MT-011的生物学特性测定结果表明：菌株致病性强菌丝体生长最适温度30℃，致死温度65℃/10min；最适pH值7；碳源以D-果糖最佳，氮源以硝酸钠最佳。产生分生孢子最适温度30℃；最适pH值9；碳源以蔗糖产孢量最大，氮源以赖氨酸产孢量最高。分生孢子萌发温度在20~40℃之间没有差异，萌发率均达90%以上；最适pH值7；光暗交替为最佳光照条件，除赖氨酸外碳源氮源的孢子萌发率高，没有差异。MT-011菌株代谢产物柱花草、辣椒和玉米的种子发芽后的根茎生长都有明显的抑制作用抑制率达83.27%，表现出很好除草活性。 马唐草新月弯孢霉Herbicidal Activity of Metabolite Produced by Curvularia lunata from Digitaria sanguinalis Yang Ye Hu Meijiao Wang Lanying (1.Environment and Plant Protection College, Hainan University, Danzhou, Hainan 571737 2.Environment and Plant Protection Institute, CATAS, Danzhou, Hainan 57173, China) Abstract： The biological characteristics of Curvularia lunata (Wakker) Boedijn (MT-011) was tested. The results showed that MT-011 displayed excellent pathoginicity. The optimum temperature for mycelial growth was 30℃ and the lethal temperature was 65℃/10min; the mycelium adapted to growon PDA whose pH value was 6; among carbon and nitrogen sources, the D- fructos and NaNO3 were best for mycelium growth. The optimum temperature for sporulation was 30℃, the optimum pH value for sporulation was 9; the sucrose and lysine were best for sporulation. The ranges of temperature for conidial germination were 20～40℃, the percentage of germination reached above 90%; the optimum pH value for germination was 7; alternative illumination was the best for germination; all carbon and nitrogen sources were better excepted lysin for germination. The active metabolite made the leaves yellow and wilting, which was very similar to the symptom caused by the pathogen itself. The metabolite exhibited excellent herbicidal activity against the indicator plant such as stylosanthes, capsicum and corn, and the inhibition percentage was 83.27%. Abstract: Curvularia lunata (Wakker) Boedijn; biological characteristics; herbicidal activity 禾本科的马唐草[Digitaria sanguinalis (L.) Scop]是世界性的恶性杂草。海南由于特殊