FGF5基因对内蒙古绒山羊绒毛性状的影响.pdfVIP

FGF5 基因对内蒙古绒山羊绒毛性状的影响 1 1 2 2 2 刘海英 ，杨桂芹 ，张微 ，朱晓萍 ，贾志海 1. 沈阳农业大学畜牧兽医学院，沈阳 110161； 2. 中国农业大学动物科技学院，农业部动物营养重点开放实验室，北京 100094 摘 要：根据FGF5 基因已知DNA序列设计了 2 对引物，采用PCR-SSCP和PCR-RFLP技术，在内蒙古绒山羊群 体中进行基因多态性检测，结果发现FGF5 基因外显子 1 存在限制性内切酶Bgl Ⅰ多态位点，对其不同基因型 个体PCR 回收产物进行测序。测序结果发现该SNP是由碱基序列C→T 的突变而引起的。基因型和基因频率统 计，该试验群体以等位基因A 具有明显的优势，χ2检验表明该SNP位点的基因频率处于Hardy-Weinberg平衡状 态；对该SNP与绒毛性状关联分析，表明该SNP对各绒毛性状的影响不显著。 关键词：内蒙古绒山羊；FGF5 ；PCR-RFLP ；PCR-SSCP ；绒毛性状 Effects of FGF5 gene on fibre traits on Inner Mongolian cashmere goats 1 1 2 2 2 LIU Hai-Ying ,YANG Gui-Qin , ZHANG Wei , ZHU Xiao-Ping , JIA Zhi-Hai 1.College of Animal Science and Veterinary Science, Shenyang Agricultureal University, Shenyang 100161, China; 2. The Key Laboratory of Animal Nutrition of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100094, China Abstract: Two pairs primers were designed base on the known sequence for amplification of FGF5 gene in the Inner Mongolian cashmere goats herd.,It was found that a restrict fragment length polymorphism within the exon 1 of FGF5 gene from the second pair of primers using PCR-SSCP and PCR-RFLP. PCR products after recovered and purified, were,sequenced that randomly selected from different genotypes .It was revealed a synonymous single base mutation (C→T) for the AB genotype. The allete A was found to be predominant in Inner Mongolian cashmere goats and the allele and genotype frequencies in our sample coincident with the Hardy-Weinberg equilibrium. The relationshios between the genotypes and fibre traits were analyzed. Fibre taits were not associated with the genotypes. Keywords ：Inner Mongolian cashmere goat; FGF5 ; PCR-RFLP; PCR-SSCP; Fibre tra