两个和盐和赤霉病菌胁迫相关的小麦糖基转移酶基因的克隆和表达.pdfVIP

HEREDITAS (Beijing) 2008 12 , 30(12): 1608―1614 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2008.01608 林凡云, 陆琼娴, 徐剑宏, 史建荣 , 210014 : 在植物体内, 糖基转移酶通过参与多种物质的糖基化而在植物抗逆境方面起着重要作用。为了解小麦糖 基转移酶基因响应病原菌和盐胁迫的分子机制, 文章分离了两个小麦糖基转移酶基因(TaUGT1, TaUGT2) 。这两 个基因均编码496 个氨基酸, cDNA 序列相似性为90 ％。它们均含有一个内含子, 分别为335 bp(TaUGT1)和324 bp( TaUGT2) 。序列比对分析表明, TaUGT1 和 TaUGT2 与尿苷二磷酸葡萄糖醛酸/ 尿苷二磷酸葡萄糖转移酶 (UDP-glucoronosyl and UDP-glucosyl transferase)基因同源性最高, 且都含有 PSPG(Putative secondary plant glycosyltransferase)保守结构域。Real-time PCR 表达分析显示, TaUGT1 受赤霉病菌抑制表达, 而TaUGT2 受赤 霉病菌诱导表达; 在高浓度NaCl 胁迫下, TaUGT1 和TaUGT2 的相对表达量分别为对照的2.87 及4.56 倍, 差异 达到极显著水平。以上结果表明, TaUGT2 可能与小麦赤霉病抗性有关, 而TaUGT1 和 TaUGT2 可能共同参与 了小麦对盐胁迫的响应。 : 小麦; 糖基转移酶; 表达分析 Cloning and expression analysis of two salt and Fusarium graminearum stress associated UDP-glucosyltransferases genes in wheat LIN Fan-Yun, LU Qiong-Xian, XU Jian-Hong, SHI Jian-Rong Institute of Food Safety Research and Inspection , Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China Abstract: Glycosyltransferases (GTs) play important roles in stress responses of plants by glycosylating hormones and secondary metabolites. UDP-glucosyltransferases (UGTs), which use UDP-glucuronic acid in animals, UDP-glucose, UDP-galactose, and UDP- rhamnose in plant as sugar donors, belong to family 1 of GTs. As a secondary metabolite pro- duced by Fusarium graminearum during infection of grains, deoxynivalenol (DON) is not only harmful to human and ani- mal’s health by inhibiting protein synthesis, but also acts as a virulence factor during fungal pathogenesis. In order to study expression profile of UGT genes in wheat, two UGTs, designated TaUGT1 and Ta