姜黄素通过抗氧化作用拮抗鱼藤酮致PC12细胞损伤研究_崔群力.pdfVIP

39 1 37 () Vol. 39 No. 1 . 37 2010 2 A cta Med Univ Sci T echnol Huazhong Feb. 2010 C12 崔群力 孙圣刚v , 430022 ( Cur) ( Ro) C12 C12 ; (MT T) ; -; ( SOD) ; DCFH-DA ( ROS) ; (Annexin Õ-FITC/ I ) C12 01 5 Lmol/ L 11 0 Lmol/ L 01 1 Lmol/ L C12 , ( P 01 01) ; ; C12 SOD , (P 01 05) ; C12 ROS , C12 , ( P 01 01) C12 , ROS, ; ; C12 ; ; R2841 1 DOI: 10. 3870/ j. issn. 1672-0741. 2010.01.010 Curcumin Antagonizes Rotenone-induced Injury of PC12 Cells by Antioxidant Acti ity v Cui Qunli, Sun Shenggang Dep artment of Neurology, Union H osp ital, Tongj i Medical College,H uaz hong University of Science and Technlogy , uhan 430022, China Abstract Objecti e T o investigate the cytoprotection of curcum in against rotenone(Ro)-induced injury and the molecular mechanisms underlying in C12 cells1 Methods The insulted model of C12 cells w as established w ith Ro1 Cell viability w as determined using MTT reduction assay1 The content of reactive oxygen species(ROS)w as determined by the method of DCFH- DA staining1 Chromatometry w as used to measure the total activity of SOD, DCFH-DA staining to measure the level of intracel- lular ROS, and flow cyt ometry to assay the apoptosis rate1 Results 01 5 and 11 0Lmol/ L curcumin significantly decreased the in- hibitory rat