【doc】桑叶提取物对糖尿病小鼠糖耐量的影响摘要.docVIP

桑叶提取物对糖尿病小鼠糖耐量的影响 《海南医学}2012年第23卷第3期HAINANMEDICALJOURNALVoi.23No.3February2012 doi:10.3969/j.issn.1003—6350.2012.03.008 桑叶提取物对糖尿病小鼠糖耐量的影响 李超,潘建明,罗新根,张相年,吴新荣 (广州军区广州总医院药剂科,广东广州510010) ? 实验研究? 【摘要】目的观察桑叶提取物(MorifoliumLeavesextract,MLE)对糖尿病小鼠分别口服3gamp;g淀粉,葡萄 糖2.4g/kg的糖耐量(GTT)的影响.方法将SPF级KM小鼠用四氧嘧啶(ALX)造成糖尿病模型,按照血糖随机分成 空白对照组,MLE高,中,低剂量组,剂量分别为180mg/kg,360mg/kg,720mg/kg,阿卡波糖对照组20mg/kg,二 甲双胍组对照组200mg/kg,ig量按30.0ml/kgBW计算,空白对照组ig给予同体积的淀粉,葡萄糖液.于给药后 不同时间测血糖,并计算血糖升高值,与空白对照组进行均数比较.结果MLE低剂量组对糖尿病小鼠口服 淀粉30min,中,高剂量组对60min,120min能显着抑制糖吸收,阿卡波糖能显着提高糖尿病小鼠的GTT.口服 葡萄糖低剂量组30min,60min中,高剂量组120min显示能抑制糖吸收,阿卡波糖对照组各时间点均不能显着 抑制糖吸收,二甲双胍阳性对照组各时间点能显着抑制糖吸收.结论MLE剂量达到180mg/kg对糖尿病小鼠 口服淀粉,葡萄糖GTT均有显着的抑制糖吸收作用.口服葡萄糖阿卡波糖对糖尿病小鼠糖耐量不产生影响,二 甲双胍能显着提高模型动物的GTT. 【关键词】桑叶提取物(MLE);糖尿病;糖耐量(GTT) 【中图分类号】R一332【文献标识码】A【文章编号】1003--6350(2012)03—024—03 Effectsofmulberryleavesextract(MLE)onglucosetolerancetestindiabeticmice.L/Chao,PANJian-ming, LUOXin—gen.ZHANGXiang—nian,Xin—rong.DepartmentofPharmacy,GuangzhouGeneralHospitalofGuangzhou MilitaryCommand,Guangzhou510010,Guangdong,CHINA 【Abstract】ObjectiveToinvestigatetheeffectsofmulberryleavesextract(MLE)onglucosetoleranceindi— abeticmiceorallyadministrated3g/kgstarchor2.4g/kgglucose.MethodsAlloxan—induceddiabeticKunming (KM)micewererandomlydividedintofourgroups:blankgroup,highMLEgroup(720mg/kgMLE),middleMLE group(360mg/kgMLE),lowMLEgroup(180mg/kgMLE),acarbosecontrolgroup(20mg/kg)andmetformincon— trolgroup(200mg/kg).Thevalueofintragastricadministrationis30.0ml/kgBW.Theblankgroupwasadministrated byequalvolumeofglucoseforinjection.Thebloodglucosewasdetected30min,60min,120minaftertreatment. Theincreasedvaluesofbloodglucosewerecalculatedandcomparedwiththeblankgroup.ResultsDiabeticmicein lowMLEgroupcouldremarkablyinhibitglucoseabsorption30minaftergivenstarch,whilethoseinmiddleMLE groupandhighMLEgroupcouldremarkablyinhibitglucoseabsorption60minand120minaftergivenstarch.Intra— gastricadministrationofacarbosetabletscouldimprovetheglucosetolerancetest(GTT)indiabeticmiceremarkably. Micein