皮肤癌相关文献习及纳米雄黄干预p53、bcl-2在a431细胞中表达的研究.pdfVIP

Skin cancer -related literature review and the study on the intervention of the expression of p53 Bcl-2 in A431 by Nanometer Realgar Speciality: Clinical internal Medicine of TCM Author: Yan qin Tutor: Professor Qi Yuanfu Abstract Objective: The study observes “Nanometer Realgar” curative effect to patients of squamous cell carcinoma (SCC) and the mechanism of inducing the apoptosis of human skin squamous cell carcinoma A431. Methods: in this experimental, mainly used immunohistochemical and RT-PCR to observe the mechanism of inducing the apopotosis of “Nanometer Realgar”to human skin squamous cell carcinoma cell line A431. Results: Nanometer Realgar can significantly induce the apoptosis of human skin squamous cell carcinoma cell line A431 by raising the level of P53 and reducing the level of Bcl- 2. Conclusion: Nanometer Realgar can induce tumor cell apoptosis ways to antitumor function, and combination therapy with DDP can produce synergy. Key words “Nanometer Realgar”; inducing apoptosis of tumor cell; Human skin squamous cell carcinoma cell line A431; Bcl-2; P53 目 录 引 言 1 第一部分 皮肤癌的理论探讨与文献复习 2 一、中医对皮肤癌的认识 2 二、皮肤鳞癌病因及病机 2 （一）紫外线照射 2 （二）人乳头瘤病毒（HPV ） 3 （三）个人因素 3 （四）其他因素 3 三、皮肤鳞癌与凋亡相关因子的研究 3 （一）Bcl-2 、Bax 与肿瘤细胞凋亡 3 （二）P53 4 （三）Survivin 与 Caspase-3 5 （四）Fas/Fasl 5 四、皮肤鳞癌的治疗 5 （一）手术治疗 5 （二）放射治疗 6 （三）光动力学疗法 6 （四）Mohs 显微切除法 6 （五）基因治疗 7 （六）药物治疗 7 （七）中医治疗 7 第二部分 纳米雄黄干预 A431 细胞的相关实验研究 8 一、一般材料与纳米雄黄溶液的制备 8 （一）一般材料 8 二、主要溶液的配制 9 三、细胞培养 10 (一)细胞培养方法 10 (二)细胞悬液的制备 11 实验一、纳米雄黄对人皮肤鳞癌 A431