行ivf-et龄妇女肾气虚的机理探讨与二至天癸颗粒疗效评估.pdfVIP

Studies on the mechanism of shen qi deficiency of senile infertile women undergoing IVF-ET and therapeutic evaluation of Erzhi Tiangui Granules Specialty: Gynecology of TCM Author: Wang Lin Tutor: Lian Fang Abstract Objective: To explore the clinical effect and mechanism of Erzhi Tiangui Granules improving the ovarian response of senile infertile women undergoing IVF-ET. Methods: In clinical study, Sixty senile infertile patients with tubal factors undergoing IVF-ET were divided into two groups at random, 30 cases in the study group treated with Erzhi Tiangui Granules and western medicine, and 30 cases in the control group were treated with gonadotropin-releasing hormone agonist (GnRH-a) and gonadotropin (Gn) alone. The short term protocol was adopted. The expression of INHB and FSH in follicular fluid on the day of retrieving oocytes were detected by ELISA and ECLIA, and symptom scores, number of retrieved oocytes, fertilization rate, high quality embryo rate and pregnancy rate were evaluated. In experimental study, 12-months old Kunming female mice whose oestrus elongation were set as primary senile female mouse model and randomized into model control group, Erzhi Tiangui Granules in high dosage, middle dosage and low dosage group. 4-months old female mice was selected as young control group additionally. The serum with decoction from primary senile mice was added to cultured granulosa cells and was incubated for 48 h.Then the concentration of estradiol progesterone and inhibin B in culture medium were measured by chemiluminescence immunoassay (CLIA) and sandwich ELISA respectively. The ovaries were treated by HE staining and In situ hybridization meth