愈溃膏对大鼠体溃疡血管生成、细胞增殖凋亡调控作用的研究.pdf

提 要 目的：观察行气活血、祛瘀生肌中药愈溃膏对大鼠体表溃疡血管生成、细胞增殖 只，参照塑料环肉芽肿定量法造模，随机分为5组，为单纯疾病模型组、生肌愈皮膏 组、愈溃膏小、中、大剂量组。分别涂药干预，观察各组肉芽肿重量，免疫组化 果：愈溃膏各剂量组均能够增加肉芽肿的重量；免疫组化显示愈溃膏提高肉芽肿组织 或O．01)；增强PCNA表达，促进创面组织细胞的增殖，中、大剂量组优于生肌愈皮 结论：行气活血、祛瘀生肌中药软膏，对模型大鼠体表溃疡有良好的愈合作用，是通 胞凋亡相关因子bel—2／bax的表达，减少细胞凋亡，发挥创面修复作用。研究从分子、 蛋白水平阐述了中药愈溃膏对体表溃疡的愈合机制，丰富了体表溃疡的实验方法及治 疗思路。 关键词体表溃疡；愈溃膏；血管内皮生长因子；碱性成纤维细胞因子；细胞增 殖／凋亡 ofEffectsofYu—kuiOintmenton and Study Angiogenesis Cell forSkinUlcerRats Proliferation／Apoptosis ofTCM Speciality：Surgery Shaobo Author：Gong Aili Tutor：Song Abstract observethe ofYu·kui interference TCMwhichcan objective：To Ointment，a drug and bloodflowandremove and regulateqi promote stagnation promotegranulation，in formationofbloodvesselsofratswitllskinulcerandincells assisting proliferation／ to the mechanismofsurface 50SPFWistar apoptosis，andstudyhealing ulcers，．Method：1 ratswereusedinthe tomakearat experiment patternbyadoptingplasticringgranuloma weredivided intofive diseasedmodel quantifying，andrandomlygroups：thesimply group， the treated and group by Ointment，three Granulation—promotingSkin·healing groups treatedYu—kuiOintmentina dose were by small，medium，largerespecti