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壮药扶芳藤对兔肌缺血再灌注损伤的保护作用及机制研究
目 录
中文摘要 ················································1
英文摘要 ················································3
引言 ·····················································6
正文 ···················································10
1 实验材料和方法 ······································10
1.1 实验材料 ·········································10
1.2 实验方法 ·········································10
1.2.1动物分组与给药方法 ····························10
1.2.2 心肌缺血再灌注损伤模型的制备 ················11
1.2.3 血清中 SOD 的检测 ·······························11
1.2.4 丙二醛（MDA ）的检测 ·····························14
1.2.5 心肌凋亡细胞原位标记的检测与分析 ··········16
1.2.6 Bax 、Bcl-2 检测方法 ···························18
1.2.7 心肌超微结构的观察 ····························19
1.2.8 统计学处理 ·····································19
2 实验结果 ············································19
2.1 各组血清 SOD 活性的测定 ··························19
2.2 各组血清 MDA 含量的测定 ·························20
2.3 各组再灌注后心肌凋亡细胞阳性指数比较 ·······22
2.4 心肌细胞 Bcl-2 、Bax 蛋白阳性表达指数比较 ·····22
2.5 心肌超微结构的观察 ······························23
３讨论 ·················································24
3.1中医对心肌缺血再灌注损伤病机的认识···················24
3.2壮药扶芳藤在临床的应用 ·······························27
3.3壮药扶芳藤对心肌缺血再灌注兔生化指标的影响 ···········28
4 存在问题与展望 ········································39
结论 ····················································39
参考文献 ················································40
附录····················································47
综述 ····················································51
致谢 ····················································72
攻读学位期间发表的学术论文目录 ··························74
广西中医学院研究生学位论文作者声明 ······················75
个 人 简 历 ·············································76
主要缩略词
英文缩写 英文全称 中文全称
apoptosis 凋亡
Evonymus fortunei Eand.- mazz.
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