转cry1Ab基因抗虫水稻的田间试验.pdfVIP

HEREDITAS (Beijing) 2007 8 , 29(8): 1008―1012 ISSN 0253-9772 研究报告 DOI: 10.1360/yc-007-1008 cry1Ab 1, 2 1 唐微 , 林拥军 1. , 430070 2. , 442000 : 采用农杆菌介导的遗传转化技术, 将Bt 基因cry1Ab 导入三系恢复系明恢63 获得了一批抗虫性及农艺性 状较好的转基因纯合株系。研究中, 进一步检测了这些纯合株系的拷贝数、Bt 蛋白含量、抗虫性及主要农艺性 状。结果显示: 5 个转基因纯合株系均为单拷贝; 纯合株系T1Ab-10 及其杂种的Bt 蛋白含量最高; 人工接虫和自 然感虫试验中, T1Ab-10 对二化螟、三化螟和稻纵卷叶螟均表现高度抗性; 喷药试验中, 阳性植株与阴性植株、 对照植株的主要农艺性状均没有显著差异。这说明转cry1Ab 基因纯合株系T1Ab-10 具商品化潜力, 可作基因聚 合材料。 : cry1Ab 基因; 抗虫性; 水稻; 田间试验 Field experiment of transgenic cry1Ab insect resistant rice TANG Wei1,2, LIN Yong-Jun1 1. National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China; 2. Research Institute of Basic Medical Science, Yunyang Medical College, Hubei Shiyan 442000, China Abstract: Gene cry1Ab was transformed into Minghui 63 (Oryza sativa L.) via Agrobacterium and several homozygous transgenic lines were obtained. In this study, the copy numbers, Bt protein contents, insect resistance, and main agronomic traits of these lines were examined. All these lines had single-copy number of cry1Ab through Southern blotting analysis. The line T1Ab-10 and its hybrid expressed the higher Cry1Ab proteins than any other lines and their corresponding hybrids when assayed by ELISA. After artificial infestation of first-instar YSB (Yellow stem borer) and natural affection of stem- borers and leaffolders, the line T1Ab-10 showed high insect-resistant to these insects. Field trial displayed that, under the pesticide-applying condition, there was no statistical difference between the negative plants of T1Ab-10 and control cultivar Minghui 6