转反义蜡质基因水稻亲本后代性状分析.pdfVIP

HEREDITAS (Beijing) 2008 9 , 30(9): 1195―1200 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2008.01195 1 1 2 1 1 1 1 刘清 , 吴顺 , 沈革志 , 彭琼 , 刘桃李 , 程鹏 , 萧浪涛 1. , 410128; 2. , 201106 摘要：以单质粒转化的粳稻广粳一号和双质粒共转化的籼稻01 早5202 所获得的转反义蜡质基因水稻后代为供 试材料, 通过潮霉素抗性、PCR 检测等手段分析了外源基因的遗传分离特性, 同时, 还分析了转基因材料的直 链淀粉含量、waxy 蛋白含量的变化特性。结果表明, 无论是采用标记基因(hpt) 与目的基因(Anti-sense waxy)连 锁的单质粒转化, 还是采用双质粒共转化, 其供试的后代植株材料都发生了外源基因分离现象, 且转基因植株 材料的直链淀粉含量都有所下降, 有些单株的直链淀粉含量已降至 10%(质量百分比) 以下, 远低于对照(其直链 淀粉含量为22.04%); SDS检测结果显示, 供试的转基因材料的waxy 蛋白的含量与对应的直链淀粉含量 呈正相关性。 ：反义蜡质基因; 水稻; 直链淀粉; 分离; waxy 蛋白 Analysis on the characters of progenies of the rice transformed with anti-sense waxy gene 1 1 2 1 1 1 LIU Qing , WU Shun , SHEN Ge-Zhi , PENG Qiong , LIU Tao-Li , CHENG Peng , 1 XIAO Lang-Tao 1. Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha 410128, China; 2. Crop Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China Abstract: The segregation of exogenous genes was studied by hygromycin-resistant and PCR experiments in the transgenic rice (Oryza sativa L. subsp. japonica and indica) with anti-sense waxy gene, meanwhile the change of amylose and waxy protein contents in progenies of transgenic rice was analyzed. The results showed no matter the rice Guangjing No.1(O. Sativa L. subsp. japonica) were transformed by p13w4 plasmid carrying anti-sense waxy gene and hygromycin-re