用EST-SSR标记对茶树种质资源的研究.pdfVIP

HEREDITAS (Beijing) 2007 1 29(1): 103 ―108 ISSN 0253-9772 研究报告 DOI: 10.1360/yc-007-0103 EST-SSR 1 1 1 2 2 金基强 , 崔海瑞 , 龚晓春 , 陈文岳, 忻雅 1. 310029; 2. , 310024 : 采用 16 对EST-SSR 引物对42 份茶树品种资源进行了分析。在这些引物中, 有 13 对可扩增出清晰条带, 其中 10 对引物具有多态性, 占76.9% 。各多态性引物的PIC 值变化范围为0.522~0.866, 平均PIC 值为0.730 。 10 对多态性引物共检测到 84 种等位基因型和 74 个等位变异, 每对引物可检测到基因型和等位基因数分别为 4~12 和3~10 种。42 份供试材料间遗传距离为 0.074~0.667, 平均遗传距离为0.363, 说明本实验所测试的材料 具有较广的遗传变异。在相似系数为0.55 的水平可将这些材料聚为3 类, 多数材料包含在第一类中。本研究表 明, 利用EST-SSR 标记进行茶树资源评价是有效的。 : 茶树; EST-SSR 标记; 种质资源分析 Studies on tea plants (Camellia sinensis) germplasms using EST- SSR marker 1 1 1 2 2 JIN Ji-Qiang , CUI Hai-Rui , GONG Xiao-Chun ,CHEN Wen-Yue , XIN Ya 1. Institute of Nuclear and Agricultural Sciences, Zhejiang University, Hangzhou 310029, China; 2. Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China Abstract: Forty two tea varieties were analyzed by using 16 SSR primer sets derived from tea ESTs in this study, and 13 of the primer sets produced clear bands and 10 of them showed polymorphism, accounting for 76.9%. The PIC (polymor- phism information content) for each polymorphic primer set varied from 0.522 to 0.866, with a average about 0.73. Totally 84 Genotypes and 74 alleles were detected in all materials by 10 polymorphic markers, with the range from 4 to 12 and from 3 to 10 for each polymorphic primer set, respectively. The genetic distance among 42 tea varieties varied from 0.074 to 0.667, averagely 0.363, suggesting that the materials used in the experiment possess a broad genetic variation. Based on th