指甲游离缘的核DNA分型研究.pdfVIP

HEREDITAS (Beijing) 2007 11 , 29(11): 1373―1377 ISSN 0253-9772 研究报告 DOI: 10.1360/yc-007-1373 DNA 1, 2 3 2 1 1 2 聂胜洁 , 杨彦梅 , 唐文如 , 许冰莹 , 景强 , 肖春杰 1. , 650031; 2. , 650091; 3. , 655000 : 为探讨指甲游离缘核DNA分型的可行性, 采集无关个体指甲游离缘样本 10 份, 分别以不同消化体系, 采 用有机法、Chelex-100 法,有机法结合Chelex-100 法等 3 种方法提取指甲游离缘核DNA, AmpFlSTR IdentifierTM试 剂盒复合扩增, ABI PRISM 3130 自动遗传分析仪检测分析。结果显示：与对照血样比较,有机法结合Chelex-100 法提取的样本核DNA均获得满意的STR分型, 有机法提取的样本核DNA 可以进行STR分型, 但部分样本图谱峰 值不均衡, Chelex-100 法提取的样本核DNA不能分型或出现较多等位基因缺失。提示指甲游离缘可以进行成功 地核DNA 的分型, 有机法和有机法结合Chelex-100 法提取的DNA 质量都可成功检测, 其中以有机法结合 Chelex-100 法提取DNA 的检测成功率最高。 : 指甲游离缘; STR; DNA 分型; DNA 提取 Extraction and analysis of nuclear DNA from free margin of nail material 1,2 3 1 2 2 NIE Sheng-Jie , YANG Yan-Mei , TANG Wen-Ru , XU Bing-Ying , JING Qiang , XIAO Chun-Jie 1 1. School of Forensic Medicine, Kunming Medical College, Kunming 650031, China ; 2. Human Genetics Center of Yunnan University, Kunming 650091, China ; 3. Institute of Criminal Science Technology of Qujing Police, Qujing 655000, China Abstract: To investigate the feasibility of DNA analysis from free margin of the nail, genomic DNA was extracted from the free margin of nail clipping of 10 volunteers using the proteinase K/SDS -based organic method, the Chelex-100 method, or a combined method. Target DNA was simultaneously amplified using a fluorescent multiplex AmpFlSTR IdentifierTM kit. The PCR products were analyzed on the ABI PRISM 3130 Genetic Analyzer. The results sh